Construct: ORF TRCN0000491509
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019657.2_s317c1
- DNA Barcode:
- GCGTTGAAGCTGGTTCCTGGGATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- OR52B2 (255725)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491509
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 255725 | OR52B2 | olfactory receptor family 5... | NM_001004052.1 | 99.8% | 99.6% | 969_970insG |
| 2 | mouse | 259063 | Olfr691 | olfactory receptor 691 | NM_147061.1 | 84.1% | 87.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1047
- ORF length:
- 972
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatgagt cacaccaatg ttaccatctt ccatcctgca gtttttgtcc 121 ttcctggcat ccctgggttg gaggcttatc acatttggct gtcaatacct ctttgcctca 181 tttacatcac tgcagtcctg ggaaacagca tcctgatagt ggttattgtc atggaacgta 241 accttcatgt gcccatgtat ttcttcctct caatgctggc cgtcatggac atcctgctgt 301 ctaccaccac tgtgcccaag gccctagcca tcttttggct tcaagcacat aacattgctt 361 ttgatgcctg tgtcacccaa ggcttctttg tccatatgat gtttgtgggg gagtcagcta 421 tcctgttagc catggccttt gatcgctttg tggccatttg tgccccactg agatatacaa 481 cagtgctaac atggcctgtt gtggggagga ttgctctggc cgtcatcacc cgaagcttct 541 gcatcatctt cccagtcata ttcttgctga agcggctgcc cttctgccta accaacattg 601 ttcctcactc ctactgtgag catattggag tggctcgttt agcctgtgct gacatcactg 661 ttaacatttg gtatggcttc tcagtgccca ttgtcatggt catcttggat gttatcctca 721 tcgctgtgtc ttactcactg atcctccgag cagtgtttcg tttgcccTCC CAGGATGCTC 781 GGCACAAGGC CCTCAGCACT TGTGGCTCCC ACCTCTGTGT CATCCTTATG TTTTATGTTC 841 CATCCTTCTT TACCTTATTG ACCCATCATT TTGGGCGTAA TATTCCTCAA CATGTCCATA 901 TCTTGCTGGC CAATCTTTAT GTGGCAGTGC CACCAATGCT GAACCCCATT GTCTATGGTG 961 TGAAGACTAA GCAGATACGT GAGGGTGTAG CCCACCGGTT CTTTGACATC AAGACTTGGT 1021 GCTGTACCTC CCCTCTGGGC TCAGACCCAG CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1081 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1141 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAG CGTTGAAGCT 1201 GGTTCCTGGG ATTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1261 att