Construct: ORF TRCN0000491540
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021334.2_s317c1
- DNA Barcode:
- CCAAGTATGCTATACAATTACTCC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- SLC1A7 (6512)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491540
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6512 | SLC1A7 | solute carrier family 1 mem... | NM_001287596.2 | 100% | 100% | |
| 2 | human | 6512 | SLC1A7 | solute carrier family 1 mem... | NM_006671.6 | 27.6% | 26.4% | (many diffs) |
| 3 | human | 6512 | SLC1A7 | solute carrier family 1 mem... | NM_001287595.2 | 24.9% | 23.9% | (many diffs) |
| 4 | human | 6512 | SLC1A7 | solute carrier family 1 mem... | NR_109858.2 | 17.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 546
- ORF length:
- 474
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggtgccg catgccatct tggcacgggg gagggacgtg tgcaggcgga 121 atggactcct catcctgtct gtgctgtctg tcatcgtggg ctgcctcctc ggcttcttct 181 tgaggacccg gcgcctctca ccacaggaaa ttagttactt ccagttccct ggagagctcc 241 TGATGAGGAT GCTGAAGATG ATGATCCTGC CACTGGTGGT CTCCAGCTTG ATGTCCGGAC 301 TTGCCTCCCT GGATGCCAAG ACCTCTAGCC GCCTGGGCGT CCTCACCGTG GCGTACTACC 361 TGTGGACCAC CTTCATGGCT GTCATCGTGG GCATCTTCAT GGTCTCCATC ATCCACCCAG 421 GCAGCGCGGC CCAGAAGGAG ACCACGGAGC AGAGTGGGAA GCCCATCATG AGCTCAGCCG 481 ATGCCCTGTT GGACCTCATC CGGCAGAAAG AAGAAAGTTG GAGGAACGGA CCAAAGGGTC 541 CTGGCTAGAA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 601 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 661 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACCAAGT ATGCTATACA ATTACTCCAC 721 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt