Construct: ORF TRCN0000491541
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016211.1_s317c1
- Derived from:
- ccsbBroadEn_15456
- DNA Barcode:
- TTTCTCGGTTCTGGTCGACCCTAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HSP90AA1 (3320)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491541
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3320 | HSP90AA1 | heat shock protein 90 alpha... | NM_005348.4 | 86.7% | 86.7% | 1_291del;1080C>T |
| 2 | human | 3320 | HSP90AA1 | heat shock protein 90 alpha... | XM_011536718.2 | 74.4% | 74.4% | 1_654del;1443C>T |
| 3 | human | 3320 | HSP90AA1 | heat shock protein 90 alpha... | NM_001017963.3 | 74.3% | 74.3% | 1_657del;1446C>T |
| 4 | mouse | 15519 | Hsp90aa1 | heat shock protein 90, alph... | NM_010480.5 | 78.6% | 85.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1971
- ORF length:
- 1905
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac caaggctgac ttgatcaata accttggtac tatcgccaag tctgggacca 121 aagcgttcat ggaagctttg caggctggtg cagatatctc tatgattggc cagttcggtg 181 ttggttttta ttctgcttat ttggttgctg agaaagtaac tgtgatcacc aaacataacg 241 atgatgagca gtacgcttgg gagtcctcag cagggggatc attcacagtg aggacagaca 301 caggtgaacc tatgggtcgt ggaacaaaag ttatcctaca cctgaaagaa gaccaaactg 361 agtacttgga ggaacgaaga ataaaggaga ttgtgaagaa acattctcag tttattggat 421 atcccattac tctttttgtg gagaaggaac gtgataaaga agtaagcgat gatgaggctg 481 aagaaaagga agacaaagaa gaagaaaaag aaaaagaaga gaaagagtcg gaagacaaac 541 ctgaaattga agatgttggt tctgatgagg aagaagaaaa gaaggatggt gacaagaaga 601 agaagaagaa gattaaggaa aagtacatcg atcaagaaga gctcaacaaa acaaagccca 661 tctggaccag aaatcccgac gatattacta atgaggagta cggagaattc tataagagct 721 tgaccaatga ctgggaagat cacttggcag tgaagcattt ttcagttgaa ggacagttgg 781 aattcagagc ccttctattt gtcccacgac gtgctccttt tgatctgttt gaaaacagaa 841 agaaaaagaa caatatcaaa ttgtatgtac gcagagtttt catcatggat aactgtgagg 901 agctaatccc tgaatatctg aacttcatta gaggggtggt agactcggag gatctccctc 961 taaacatatc ccgtgagatg ttgcaacaaa gcaaaatttt gaaagttatc aggaagaatt 1021 tggtcaaaaa atgcttagaa ctctttactg aactggcgga agataaagag aactacaaga 1081 aattctatga gcagttctct aaaaacataa agcttggaat acacgaagac tctcaaaatc 1141 ggaagaagct ttcagagctg ttaaggtact acacatctgc ctctggtgat gagatggttt 1201 ctctcaagga ctactgcacc agaatgaagg agaaccagaa acatatctat tatatcacag 1261 gtgagaccaa ggaccaggta gctaactcag cctttgtgga acgtcttcgg aaacatggct 1321 tagaagtgat ctatatgatt gagcccattg atgagtactg tgtccaacag ctgaaggaat 1381 ttgaggggaa gactttagtg tcagtcacca aagaaggcct ggaacttcca gaggatgaag 1441 aagagaaaaa gaagcaggaa gagaaaaaaa caaagtttga gaacctctgc aaaatcatga 1501 aagacatatt ggagaaaaaa gttgaaaagg tggttgtgtc aaaccgattg gtgacatctc 1561 catgctgtat tgtcacaagc acatatggct ggacagcaaa catggagaga atcatgaaag 1621 ctcaagccct aagagacaac tcaacaatgg gttacatggc agcaaagaaa cacctggaga 1681 taaaccctga ccattccatt attgagacct taaggcaaaa ggcagaggct gataagaacg 1741 acaagtctgt gaaggatctg gtcatcttgc tttatgaaac tgcgctcctg tcttctggct 1801 tcagtctgga agatccccag acacatgcta acaggatcTA CAGGATGATC AAACTTGGTC 1861 TGGGTATTGA TGAAGATGAC CCTACTGCTG ATGATACCAG TGCTGCTGTA ACTGAAGAAA 1921 TGCCACCCCT TGAAGGAGAT GACGACACAT CACGCATGGA AGAAGTAGAC TACCCAACTT 1981 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 2041 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 2101 CTTGTGGAAA GGACGATTTC TCGGTTCTGG TCGACCCTAT ACGCGTTAAG TCgacaatca 2161 acctctggat tacaaaattt gtgaaagatt