Construct: ORF TRCN0000491545
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012586.1_s317c1
- Derived from:
- ccsbBroadEn_15411
- DNA Barcode:
- ATACGAGACTGAATGCTTGTGCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ETS2 (2114)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491545
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2114 | ETS2 | ETS proto-oncogene 2, trans... | NM_005239.6 | 99.7% | 100% | 816T>G;1023A>G;1395C>A |
| 2 | human | 2114 | ETS2 | ETS proto-oncogene 2, trans... | XM_005260935.1 | 99.7% | 100% | 816T>G;1023A>G;1395C>A |
| 3 | human | 2114 | ETS2 | ETS proto-oncogene 2, trans... | XM_017028290.1 | 99.7% | 100% | 816T>G;1023A>G;1395C>A |
| 4 | human | 2114 | ETS2 | ETS proto-oncogene 2, trans... | NM_001256295.2 | 76.8% | 77% | (many diffs) |
| 5 | mouse | 23872 | Ets2 | E26 avian leukemia oncogene... | NM_011809.3 | 85.5% | 91.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1473
- ORF length:
- 1407
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa tgatttcgga atcaagaata tggaccaggt agcccctgtg gctaacagtt 121 acagagggac actcaagcgc cagccagcct ttgacacctt tgatgggtcc ctgtttgctg 181 tttttccttc tctaaatgaa gagcaaacac tgcaagaagt gccaacaggc ttggattcca 241 tttctcatga ctccgccaac tgtgaattgc ctttgttaac cccgtgcagc aaggctgtga 301 tgagtcaagc cttaaaagct accttcagtg gcttcaaaaa ggaacagcgg cgcctgggca 361 ttccaaagaa cccctggctg tggagtgagc aacaggtatg ccagtggctt ctctgggcca 421 ccaatgagtt cagtctggtg aacgtgaatc tgcagaggtt cggcatgaat ggccagatgc 481 tgtgtaacct tggcaaggaa cgctttctgg agctggcacc tgactttgtg ggtgacattc 541 tctgggaaca tctggagcaa atgatcaaag aaaaccaaga aaagacagaa gatcaatatg 601 aagaaaattc acacctcacc tccgttcctc attggattaa cagcaataca ttaggttttg 661 gcacagagca ggcgccctat ggaatgcaga cacagaatta ccccaaaggc ggcctcctgg 721 acagcatgtg tccggcctcc acacccagcg tactcagctc tgagcaggag tttcagatgt 781 tccccaagtc tcggctcagc tccgtcagcg tcacctactg ctctgtcagt caggacttcc 841 caggcagcaa cttgaatttg ctcaccaaca attctgggac gcccaaagac cacgactccc 901 ctgagaacgg tgcggacagc ttcgagagct cagactccct cctccagtcc tggaacagcc 961 agtcgtcctt gctggatgtg caacgggttc cttccttcga gagcttcgaa gatgactgca 1021 gccagtctct ctgcctcaat aagccaacca tgtctttcaa ggattacatc caagagagga 1081 gtgacccggt ggagcaaggc aaaccagtta tacctgcagc tgtgctggcc ggcttcacag 1141 gaagtggacc tattcagctg tggcagtttc tcctGGAGCT GCTATCAGAC AAATCCTGCC 1201 AGTCATTCAT CAGCTGGACT GGAGACGGAT GGGAGTTTAA GCTCGCCGAC CCCGATGAGG 1261 TGGCCCGCCG GTGGGGAAAG AGGAAAAATA AGCCCAAGAT GAACTACGAG AAGCTGAGCC 1321 GGGGCTTACG CTACTATTAC GACAAGAACA TCATCCACAA GACGTCGGGG AAGCGCTACG 1381 TGTACCGCTT CGTGTGCGAC CTCCAGAACT TGCTGGGGTT CACGCCCGAG GAACTGCACG 1441 CCATCCTGGG CGTCCAGCCA GACACGGAGG ACTGCCCAAC TTTCTTGTAC AAAGTGGTTG 1501 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1561 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAT 1621 ACGAGACTGA ATGCTTGTGC GCACGCGTTA AGTCgacaat caacctctgg attacaaaat 1681 ttgtgaaaga tt