Construct: ORF TRCN0000491556
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006365.1_s317c1
- Derived from:
- ccsbBroadEn_07560
- DNA Barcode:
- CGATCATATGACCGTTTTGCTACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RBM12 (10137)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491556
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10137 | RBM12 | RNA binding motif protein 12 | NM_001198838.2 | 99.9% | 100% | 228G>A |
| 2 | human | 10137 | RBM12 | RNA binding motif protein 12 | NM_001198840.2 | 99.9% | 100% | 228G>A |
| 3 | human | 10137 | RBM12 | RNA binding motif protein 12 | NM_006047.6 | 99.9% | 100% | 228G>A |
| 4 | human | 10137 | RBM12 | RNA binding motif protein 12 | NM_152838.4 | 99.9% | 100% | 228G>A |
| 5 | mouse | 75710 | Rbm12 | RNA binding motif protein 12 | NM_029397.3 | 84.7% | 86.7% | (many diffs) |
| 6 | mouse | 75710 | Rbm12 | RNA binding motif protein 12 | NM_170598.2 | 84.7% | 86.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2862
- ORF length:
- 2796
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgtggtcatc cgtttgcaag gtctcccaat tgtggcgggg accatggaca 121 ttcgccactt cttctctgga ttgaccattc ctgatggggg cgtgcatatt gtagggggtg 181 aactgggtga ggctttcatc gtttttgcca ctgatgaaga tgcaaggctt ggtatgatgc 241 gcacaggtgg tacaattaaa gggtcaaaag taacactatt gttgagtagt aaaacggaaa 301 tgcagaatat gattgaactg agtcgtaggc gttttgaaac tgccaactta gatataccac 361 cagcaaatgc cagtagatca ggaccaccac ctagctcagg aatgagtagc agggtaaact 421 tgcccacaac agtatccaac tttaataatc catcacccag tgtagttact gccaccactt 481 ctgttcatga aagcaacaaa aacatacaga cattttccac agccagcgta ggaacagctc 541 ctccaaatat gggggcttcc tttgggagcc caacgtttag ctcaactgtt ccaagcacag 601 cctctccaat gaacacagtc ccgccgccac caattcctcc aattccagcg atgccatctc 661 tgccaccaat gccatccatt cccccaattc cagttcctcc tccagtacct acattgcctc 721 ctgtgcctcc tgtgcccccg attcccccag ttccttctgt gccacccatg accccactgc 781 cacccatgtc gggcatgccg cccttgaatc cgccacctgt ggcacctcta cctgctggaa 841 tgaatggctc tggagcacct atgaatttga acaataatct gaatcctatg tttcttggtc 901 cgttgaatcc tgttaaccct atccagatga actctcagag cagtgtgaag ccactcccca 961 tcaaccctga tgatctgtat gtcagtgtgc atggaatgcc cttttctgca atggaaaatg 1021 atgtcagaga tttttttcat gggctccgtg ttgatgcagt gcatttgttg aaagatcatg 1081 taggtcgaaa taatgggaat ggattggtta agtttctctc ccctcaagat acatttgaag 1141 ctttgaaacg aaacagaatg ctgatgattc aacgctatgt ggaagttagc cctgccacag 1201 aaagacagtg ggtagctgct ggaggccata tcacttttaa gcaaaatatg ggaccttctg 1261 gacaaactca tccccctcct cagacacttc ccaggtcaaa atcgcccagt gggcagaaaa 1321 gatcaaggtc aagatcacca catgaggctg gtttttgtgt ttacttgaaa gggctaccat 1381 ttgaagcaga aaacaaacat gtcattgatt tttttaaaaa gctggatatt gtggaagata 1441 gtatttatat agcttatgga cccaatggga aagcaactgg cgaaggcttt gtagagttca 1501 gaaatgaggc tgactataag gctgctctgt gtcgtcataa acagtacatg ggcaatcgct 1561 ttattcaagt tcatccaatt actaagaaag gtatgctaga aaagatagat atgattcgaa 1621 aaagactgca gaacttcagc tatgaccaga gggaaatgat actaaatcca gagggggatg 1681 tcaactctgc caaagtctgt gcccacataa caaatattcc attcagcatt acaaagatgg 1741 atgttcttca gttcctagaa ggaatcccag tggatgaaaa tgctgtacat gttcttgttg 1801 ataacaatgg gcaaggtcta ggacaggcat tggttcagtt taaaaatgaa gatgatgcac 1861 gtaagtctga acgcttacac cgtaaaaaac ttaatgggag agaagctttt gttcatgtag 1921 ttaccctaga agatatgaga gagattgaga aaaatccccc tgcccaagga aaaaagggat 1981 taaagatgcc tgtgccaggt aatcctgcag ttccaggaat gcccaatgcg ggactgcccg 2041 gtgtgggact gcccagtgca ggacttcccg gtgcaggcct gcccagcaca ggactgcctg 2101 gttcagcaat aaccagtgca ggactgcctg gtgcgggaat gcccagtgca ggaataccta 2161 gtgcaggagg tgaagagcat gccttcctga ctgtaggatc aaaggaagcc aataatgggc 2221 ctccatttaa ctttcctggt aattttggtg gatcaaatgc ctttgggcca ccaatccctc 2281 ctccaggatt aggaggcggg gcctttggtg atgctaggcc tggtatgcct tcagttggaa 2341 acagtggttt gcctggtcta ggactggatg ttccgggttt tggaggtgga ccaaacaatt 2401 taagtgggcc atcgggattt ggagggggcc ctcagaattt tggaaatggc cctggtagct 2461 taggcggtcc cccggggttt ggaagtggcc ctcctggtct tGGAAGTGCC CCTGGGCATT 2521 TGGGTGGGCC ACCAGCTTTT GGGCCTGGCC CCGGCCCCGG CCCCGGCCCT GGCCCAATCC 2581 ATATTGGTGG TCCCCCTGGC TTTGCATCTA GTTCTGGAAA ACCAGGACCG ACAGTAATTA 2641 AAGTGCAAAA CATGCCCTTT ACTGTGTCTA TTGATGAGAT TTTAGATTTC TTTTATGGCT 2701 ATCAAGTAAT CCCAGGCTCA GTGTGTTTAA AATACAATGA AAAAGGTATG CCCACAGGTG 2761 AAGCCATGGT GGCCTTTGAG TCTCGGGATG AAGCCACAGC TGCTGTCATT GACTTAAATG 2821 ACAGGCCTAT AGGTTCAAGA AAAGTAAAAC TTGTATTAGG GTACCCAACT TTCTTGTACA 2881 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 2941 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 3001 AGGACGACGA TCATATGACC GTTTTGCTAC GACGCGTTAA GTCgacaatc aacctctgga 3061 ttacaaaatt tgtgaaagat t