Construct: ORF TRCN0000491562
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010355.2_s317c1
- Derived from:
- ccsbBroadEn_06987
- DNA Barcode:
- GGTCACGCCCGACAAAGTAAGCAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SPAM1 (6677)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491562
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6677 | SPAM1 | sperm adhesion molecule 1 | NM_003117.5 | 99.9% | 100% | 582G>A |
| 2 | human | 6677 | SPAM1 | sperm adhesion molecule 1 | NM_001174044.2 | 97.2% | 97.2% | (many diffs) |
| 3 | human | 6677 | SPAM1 | sperm adhesion molecule 1 | NM_001174045.1 | 97.2% | 97.2% | (many diffs) |
| 4 | human | 6677 | SPAM1 | sperm adhesion molecule 1 | NM_001174046.1 | 97.2% | 97.2% | (many diffs) |
| 5 | human | 6677 | SPAM1 | sperm adhesion molecule 1 | NM_153189.3 | 97.2% | 97.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1599
- ORF length:
- 1533
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg agtgctaaaa ttcaagcaca tctttttcag aagctttgtt aaatcaagtg 121 gagtatccca gatagttttc accttccttc tgattccatg ttgcttgact ctgaatttca 181 gagcacctcc tgttattcca aatgtgcctt tcctctgggc ctggaatgcc ccaagtgaat 241 tttgtcttgg aaaatttgat gagccactag atatgagcct cttctctttc ataggaagcc 301 cccgaataaa cgccaccggg caaggtgtta caatatttta tgttgataga cttggctact 361 atccttacat agattcaatc acaggagtaa ctgtgaatgg aggaatcccc cagaagattt 421 ccttacaaga ccatctggac aaagctaaga aagacattac attttatatg ccagtagaca 481 atttgggaat ggctgttatt gactgggaag aatggagacc cacttgggca agaaactgga 541 aacctaaaga tgtttacaag aataggtcta ttgaattggt tcagcaacaa aatgtacaac 601 ttagtctcac agaggccact gagaaagcaa aacaagaatt tgaaaaagca gggaaggatt 661 tcctggtaga gactataaaa ttgggaaaat tacttcggcc aaatcacttg tggggttatt 721 atctttttcc ggattgttac aaccatcact ataagaaacc cggttacaat ggaagttgct 781 tcaatgtaga aataaaaaga aatgatgatc tcagctggtt gtggaatgaa agcactgctc 841 tttacccatc catttatttg aacactcagc agtctcctgt agctgctaca ctctatgtgc 901 gcaatcgagt tcgggaagcc atcagagttt ccaaaatacc tgatgcaaaa agtccacttc 961 cggtttttgc atatacccgc atagttttta ctgatcaagt tttgaaattc ctttctcaag 1021 atgaacttgt gtatacattt ggcgaaactg ttgctctggg tgcttctgga attgtaatat 1081 ggggaaccct cagtataatg cgaagtatga aatcttgctt gctcctagac aattacatgg 1141 agactatact gaatccttac ataatcaacg tcacactagc agccaaaatG TGTAGCCAAG 1201 TGCTTTGCCA GGAGCAAGGA GTGTGTATAA GGAAAAACTG GAATTCAAGT GACTATCTTC 1261 ACCTCAACCC AGATAATTTT GCTATTCAAC TTGAGAAAGG TGGAAAGTTC ACAGTACGTG 1321 GAAAACCGAC ACTTGAAGAC CTGGAGCAAT TTTCTGAAAA ATTTTATTGC AGCTGTTATA 1381 GCACCTTGAG TTGTAAGGAG AAAGCTGATG TAAAAGACAC TGATGCTGTT GATGTGTGTA 1441 TTGCTGATGG TGTCTGTATA GATGCTTTTC TAAAACCTCC CATGGAGACA GAAGAACCTC 1501 AAATTTTCTA CAATGCTTCA CCCTCCACAC TATCTGCCAC AATGTTCATT TGGAGGCTGG 1561 AAGTCTGGGA TCAAGGTATT AGCAGAATTG GTTTCTTCCG CCCAACTTTC TTGTACAAAG 1621 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1681 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1741 ACGAGGTCAC GCCCGACAAA GTAAGCAAAC GCGTTAAGTC gacaatcaac ctctggatta 1801 caaaatttgt gaaagatt