Construct: ORF TRCN0000491564
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020521.2_s317c1
- DNA Barcode:
- AAGCACTAGAATGTAGCCCACATC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- UCK1 (83549)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491564
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 83549 | UCK1 | uridine-cytidine kinase 1 | NM_001135954.2 | 99.8% | 99.5% | 538G>A |
| 2 | human | 83549 | UCK1 | uridine-cytidine kinase 1 | NM_031432.5 | 72.4% | 62.5% | 508_602del;633G>A;699_831del |
| 3 | human | 83549 | UCK1 | uridine-cytidine kinase 1 | NM_001261451.2 | 69.7% | 58.5% | (many diffs) |
| 4 | human | 83549 | UCK1 | uridine-cytidine kinase 1 | XR_246602.4 | 69.4% | (many diffs) | |
| 5 | human | 83549 | UCK1 | uridine-cytidine kinase 1 | NM_001261450.2 | 69.1% | 59.2% | (many diffs) |
| 6 | human | 83549 | UCK1 | uridine-cytidine kinase 1 | XM_005272224.2 | 68.9% | 59.5% | (many diffs) |
| 7 | human | 83549 | UCK1 | uridine-cytidine kinase 1 | NM_001318519.1 | 64.1% | 54.2% | (many diffs) |
| 8 | human | 83549 | UCK1 | uridine-cytidine kinase 1 | NR_134666.1 | 26.2% | (many diffs) | |
| 9 | human | 83549 | UCK1 | uridine-cytidine kinase 1 | NR_134667.1 | 26.1% | (many diffs) | |
| 10 | human | 83549 | UCK1 | uridine-cytidine kinase 1 | XR_002956811.1 | 22.5% | (many diffs) | |
| 11 | mouse | 22245 | Uck1 | uridine-cytidine kinase 1 | NM_011675.2 | 62.5% | 56.9% | (many diffs) |
| 12 | mouse | 22245 | Uck1 | uridine-cytidine kinase 1 | XM_006497853.1 | 53.6% | 48.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 675
- ORF length:
- 603
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggcttcg gcgggaggcg aagactgcga gagccccgcg ccggaggccg 121 accgtccgca ccagcggccc ttcctgatag gggtgagcgg cggcactgcc agcgggaagt 181 cgaccgtgtg tgagaagatc atggagttgc tgggacagaa cgaggtggaa cagcggcagc 241 ggaaggtggt catccTGAGC CAGGACAGGT TCTACAAGGT CCTGACGGCA GAGCAGAAGG 301 CCAAGGCCTT GAAAGGACAG TACAATTTTG ACCATCCAGA TGCCTTTGAT AATGATTTGA 361 TGCACAGGAC TCTGAAGAAC ATCGTGGAGG GCAAAACGGT GGAGGTGCCG ACCTATGATT 421 TTGTGACACA CTCAAGGTTA CCAGAGACCA CGGTGGTCTA CCCTGCGGAC GTGGTTCTGT 481 TTGAGGGCAT CTTGGTGTTC TACAGCCAGG AGATCCGGGA CATGTTCCAC CTGCGCCTCT 541 TCGTGGACAC CGACTCCGAC GTCAGGCTGT CTCGAAGAGA CAAAGAAGTA TGCCGATGTG 601 ATCATCCCAC GAGGAGTGGA CAATATGGTT GCCATCAACC TGATCGTGCA GCACATCCAG 661 GACATTCTGA ATGGTAGAAC CCAGCTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 721 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 781 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAAAGCACT AGAATGTAGC 841 CCACATCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt