Construct: ORF TRCN0000491577
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021677.2_s317c1
- DNA Barcode:
- TGTGCAATGTCACGCGCGTACGCT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- CYP11A1 (1583)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491577
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1583 | CYP11A1 | cytochrome P450 family 11 s... | NM_000781.3 | 100% | 100% | |
| 2 | human | 1583 | CYP11A1 | cytochrome P450 family 11 s... | NM_001099773.2 | 69.6% | 69.6% | 0_1ins474 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1632
- ORF length:
- 1563
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gctggccaag ggtcttcccc cacgctcagt cctggtcaaa ggctgccaga 121 cctttctgag tgcccccagg gaggggctgg ggcgtctcag ggtgcccact ggcgagggag 181 ctggcatctc cacccgcagt cctcgcccct tcaatgagat cccctctcct ggtgacaatg 241 gctggctaaa cctgtaccat ttctggaggg agacgggcac acacaaagtc caccttcacc 301 atgtccagaa tttccagaag tatggcccga tttacaggga gaagctcggc aacgtggagt 361 cggtttatgt catcgaccct gaagatgtgg cccttctctt taagtccgag ggccccaacc 421 cagaacgatt cctcatcccg ccctgggtcg cctatcacca gtattaccag agacccatag 481 gagtcctgtt gaagaagtcg gcagcctgga agaaagaccg ggtggccctg aaccaggagg 541 tgatggctcc agaggccacc aagaactttt tgcccctgtt ggatgcagtg tctcgggact 601 tcgtcagtgt cctgcacagg cgcatcaaga aggcgggctc cggaaattac tcgggggaca 661 tcagtgatga cctgttccgc tttgcctttg agtccatcac taacgtcatt tttggggagc 721 gccaggggat gctggaggaa gtagtgaacc ccgaggccca gcgattcatt gatgccatct 781 accagatgtt ccacaccagc gtccccatgc tcaaccttcc cccagacctg ttccgtctgt 841 tcaggaccaa gacctggaag gaccatgtgg ctgcatggga cgtgattttc agtaaagctg 901 acatatacac ccagaacttc tactgggaat tgagacagaa aggaagtgtt caccacgatt 961 accgtggcat cctctacaga ctcctgggag acagcaagat gtccttcgag gacatcaagg 1021 ccaacgtcac agagatgctg gcaggagggg tggacacgac gtccatgacc ctgcagtggc 1081 acttgtatga gatggcacgc aacctgaagg tgcaggatat gctgcgggca gaggtcttgg 1141 ctgcgcggca ccaggcccag ggagacatgg ccacgatgct acagctggtc cccctcctca 1201 aagccagcat caaggagaca ctaagacttc accccatctc cgtgaccctg cagagatatc 1261 ttgtaaatga cttggttctt cgagattaca tgattcctgc caagacactg gtgcaagtgg 1321 ccatctatgc tctgggccga gagcccacct tcTTCTTCGA CCCGGAAAAT TTTGACCCAA 1381 CCCGATGGCT GAGCAAAGAC AAGAACATCA CCTACTTCCG GAACTTGGGC TTTGGCTGGG 1441 GTGTGCGGCA GTGTCTGGGA CGGCGGATCG CTGAGCTAGA GATGACCATC TTCCTCATCA 1501 ATATGCTGGA GAACTTCAGA GTTGAAATCC AACACCTCAG CGATGTGGGC ACCACATTCA 1561 ACCTCATTCT GATGCCTGAA AAGCCCATCT CCTTCACCTT CTGGCCCTTT AACCAGGAAG 1621 CAACCCAGCA GTAGAACCCA GCTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1681 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1741 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TGTGCAATGT CACGCGCGTA 1801 CGCTACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt