Construct: ORF TRCN0000491578
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016888.2_s317c1
- Derived from:
- ccsbBroadEn_05189
- DNA Barcode:
- AATCTAAAATACGTGACCGAACCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- APLF (200558)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491578
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 200558 | APLF | aprataxin and PNKP like factor | NM_173545.3 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1599
- ORF length:
- 1533
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc cgggggcttc gagctgcagc cgcgggacgg cggtccccgg gtggccctgg 121 cgcccgggga gacggtgatc ggccgcgggc cgctgctggg aataacagac aagagagtat 181 ccagaagaca tgccattctt gaggtggcag gtggtcagct gcgaatcaaa ccgatacaca 241 caaatccatg tttttaccag tcttcagaga agagtcagct cttaccattg aagccaaatc 301 tatggtgcta tttgaatcct ggagacagct tttctttgtt agttgacaaa tacattttcc 361 gcattctctc tataccctct gaagtggaaa tgcaatgtac cttaagaaac agtcaagtgc 421 ttgatgaaga taatatattg aatgaaacac caaaatcccc cgtgattaat ttacctcatg 481 agactactgg tgcctcacaa ctggaaggaa gcacagaaat agccaagacc cagatgactc 541 ccacaaatag tgtgtctttc ctaggtgaaa atagagactg caataagcag cagccaatcc 601 ttgccgagag gaaaagaatc cttccaactt ggatgttagc agaacattta agtgatcaaa 661 acctttcagt accagcaatc agtggaggta atgtaatcca gggaagtgga aaagaagaaa 721 tctgcaaaga taaatcccag ctaaacacaa cccagcaagg aagaaggcaa ttaatttcat 781 caggaagttc agaaaataca tcagcagaac aagacacagg agaagagtgc aaaaatactg 841 atcaggaaga gtctaccatt tcatccaagg aaatgccaca atcattttct gcaatcacat 901 taagtaacac agagatgaat aatattaaga ctaatgcaca gagaaacaaa cttccaatag 961 aggaacttgg taaagtttct aaacataaaa ttgccactaa aagaacacca cataaagaag 1021 atgaggcaat gagctgttct gaaaattgtt cgagtgccca gggcgactca cttcaggatg 1081 agtctcaagg gtctcattct gagtccagct ctaatccctc caatcctgaa actttgcatg 1141 caaaggcaac tgattcagtt ctacaaggtt ctgaaggaaa caaggtcaag aggacatcct 1201 gcatgtatgg ggcaaactgc tataggaaga atcctgttca ttttcaacat tttagccatc 1261 ctggtgatag tgattatGGA GGTGTACAAA TCGTGGGCCA AGATGAGACT GATGACCGGC 1321 CTGAATGTCC CTATGGACCA TCCTGTTATA GGAAGAATCC CCAGCACAAG ATAGAATATA 1381 GACATAATAC GCTTCCAGTG AGAAATGTTT TAGATGAAGA TAATGATAAT GTTGGGCAAC 1441 CCAATGAGTA TGACCTGAAC GACAGCTTTC TAGATGATGA GGAAGAAGAC TATGAGCCAA 1501 CAGATGAAGA TTCTGACTGG GAACCAGGAA AGGAAGATGA AGAGAAGGAA GATGTGGAAG 1561 AGCTTTTGAA AGAAGCAAAA AGGTTTATGA AAAGAAAATA CCCAACTTTC TTGTACAAAG 1621 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1681 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1741 ACGAAATCTA AAATACGTGA CCGAACCAAC GCGTTAAGTC gacaatcaac ctctggatta 1801 caaaatttgt gaaagatt