Construct: ORF TRCN0000491586
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020487.2_s317c1
- DNA Barcode:
- AAAAACGGGAGTGAGAGGCTGGTA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- STK40 (83931)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491586
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 83931 | STK40 | serine/threonine kinase 40 | NM_001282547.2 | 99.9% | 100% | 1212C>T |
| 2 | human | 83931 | STK40 | serine/threonine kinase 40 | NM_032017.3 | 99.9% | 100% | 1212C>T |
| 3 | human | 83931 | STK40 | serine/threonine kinase 40 | NM_001282546.2 | 98.7% | 98.8% | 112_126del;1227C>T |
| 4 | mouse | 74178 | Stk40 | serine/threonine kinase 40 | NM_028800.3 | 89.7% | 97.2% | (many diffs) |
| 5 | mouse | 74178 | Stk40 | serine/threonine kinase 40 | XM_006503442.1 | 89.7% | 97.2% | (many diffs) |
| 6 | mouse | 74178 | Stk40 | serine/threonine kinase 40 | XM_017320414.1 | 89.7% | 97.2% | (many diffs) |
| 7 | mouse | 74178 | Stk40 | serine/threonine kinase 40 | NM_001145827.1 | 86.9% | 94.2% | (many diffs) |
| 8 | mouse | 74178 | Stk40 | serine/threonine kinase 40 | XM_006503441.3 | 86.9% | 94.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1377
- ORF length:
- 1305
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgaagcgg agagcatcag acagaggagc tggggaaacg tcggccaggg 121 ccaaggctct aggaagtggg atttctggaa ataatgcaaa gagagctgga ccattcatcc 181 ttggtccccg tctgggcaac tcaccggtgc caagcatagt gcagtgtttg gcgaggaaag 241 atggcacgga tgacttctat cagctgaaga tcctgaccct ggaggagagg ggggaccaag 301 gcatagagag ccaggaagag cggcagggca agatgctgct gcacaccgag tactcactgc 361 tgtctctcct gcacacgcag gatggcgtgg tgcaccacca cggcctcttc caggaccgca 421 cctgtgaaat cgttgaggac acagaatcca gccggatggt taagaagatg aagaagcgca 481 tctgcctcgt cctggactgc ctctgtgctc atgacttcag cgataagacc gctgacctca 541 tcaacctgca gcactacgtc atcaaggaga agaggctcag cgagagggag actgtggtaa 601 tcttctacga cgtggtccgc gtggtggagg ccctgcacca gaaaaatatc gtgcacagag 661 acctgaagct ggggaacatg gtgctcaaca agaggacaca tcggataacc atcaccaact 721 tctgcctcgg gaagcatctg gtgagcgagg gggacctgct gaaggaccag agagggagcc 781 ctgcctacat cagtcccgac gtgctcagcg gccggccgta ccgtggcaag cccagtgaca 841 tgtgggccct gggcgtggtg ctcttcacca tgctgtatgg ccagttcccc ttctacgaca 901 gcatcccgca ggagctcttc cgcaagatca aggctgccga gtataccatt cctgaggatg 961 gacgGGTTTC TGAGAACACC GTGTGTCTCA TCCGGAAGCT GCTGGTCCTT GACCCCCAGC 1021 AGCGCCTGGC CGCCGCCGAC GTCCTGGAGG CCCTCAGTGC CATCATTGCA TCATGGCAGT 1081 CCCTGTCATC TCTGAGTGGG CCTTTGCAAG TGGTTCCTGA CATTGATGAC CAAATGAGCA 1141 ATGCGGATAG CTCCCAGGAG GCGAAGGTGA CGGAGGAGTG CTCCCAGTAC GAGTTTGAGA 1201 ACTACATGCG TCAGCAGCTG CTGCTGGCCG AGGAGAAGAG CTCCATCCAT GACGCCCGGA 1261 GCTGGGTACC CAAGCGGCAG TTTGGCAGCG CACCACCGGT GCGACGGCTG GGCCACGACG 1321 CACAGCCCAT GACCTCCTTG GACACGGCCA TCCTGGCGCA GCGCTACCTG CGGAAATAGG 1381 ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1441 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTCGA TTTCTTGGCT 1501 TTATATATCT TGTGGAAAGG ACGAAAAAAC GGGAGTGAGA GGCTGGTAAC GCGTTAAGTC 1561 gacaatcaac ctctggatta caaaatttgt gaaagatt