Transcript: Mouse NM_001145827.1

Mus musculus serine/threonine kinase 40 (Stk40), transcript variant 1, mRNA.

Source:
NCBI, updated 2017-06-04
Taxon:
Mus musculus (mouse)
Gene:
Stk40 (74178)
Length:
3877
CDS:
511..1860

Additional Resources:

NCBI RefSeq record:
NM_001145827.1
NBCI Gene record:
Stk40 (74178)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001145827.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000322271 AGCGCGAGACTGTGGTCATTT pLKO_005 1064 CDS 100% 13.200 18.480 N Stk40 n/a
2 TRCN0000220727 CACAACGCTATCTGCGGAAAT pLKO.1 1838 CDS 100% 10.800 15.120 N Stk40 n/a
3 TRCN0000322267 CACAACGCTATCTGCGGAAAT pLKO_005 1838 CDS 100% 10.800 15.120 N Stk40 n/a
4 TRCN0000322269 AGTGCTCCCAATACGAGTTTG pLKO_005 1658 CDS 100% 10.800 8.640 N Stk40 n/a
5 TRCN0000322270 TGCAAGTGGTTCCGGACATTG pLKO_005 1586 CDS 100% 10.800 8.640 N Stk40 n/a
6 TRCN0000322268 ATTCTAGATAGATAGCTAATC pLKO_005 2083 3UTR 100% 10.800 7.560 N Stk40 n/a
7 TRCN0000220728 CGGACATTGATGACCAAATGA pLKO.1 1598 CDS 100% 5.625 3.938 N Stk40 n/a
8 TRCN0000220726 CCAATACGAGTTTGAGAACTA pLKO.1 1665 CDS 100% 4.950 3.465 N Stk40 n/a
9 TRCN0000220725 CCATCGGATAACTATCACCAA pLKO.1 1179 CDS 100% 2.640 1.848 N Stk40 n/a
10 TRCN0000220729 CCCGCCTACATCAGTCCTGAT pLKO.1 1261 CDS 100% 1.350 0.945 N Stk40 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001145827.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04308 pDONR223 100% 87% 94.2% None (many diffs) n/a
2 ccsbBroad304_04308 pLX_304 0% 87% 94.2% V5 (many diffs) n/a
3 TRCN0000480740 TCTGCGAACATCTCTTTCGATATC pLX_317 29.9% 87% 94.2% V5 (many diffs) n/a
4 ccsbBroadEn_15186 pDONR223 0% 87% 94.2% None (many diffs) n/a
5 ccsbBroad304_15186 pLX_304 0% 87% 94.2% V5 (many diffs) n/a
6 TRCN0000480161 TCCCTGTCGTGTCATCGCCACGTA pLX_317 23.9% 87% 94.2% V5 (many diffs) n/a
7 TRCN0000491586 AAAAACGGGAGTGAGAGGCTGGTA pLX_317 31.9% 86.9% 94.2% V5 (not translated due to prior stop codon) (many diffs) n/a
8 TRCN0000489086 CTGCCGTGATAGCGGAGACATAAC pLX_317 26.2% 86.8% 94% V5 (many diffs) n/a
9 ccsbBroadEn_12762 pDONR223 100% 46.8% 50.5% None (many diffs) n/a
10 ccsbBroad304_12762 pLX_304 0% 46.8% 50.5% V5 (many diffs) n/a
11 TRCN0000478606 GCTGACTAAATAAGGCGCGCCACG pLX_317 41% 46.8% 50.5% V5 (many diffs) n/a
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