Construct: ORF TRCN0000491593
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015289.1_s317c1
- Derived from:
- ccsbBroadEn_06426
- DNA Barcode:
- GCTTCTTGCCTGACCTTGTTTTGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IFIT1 (3434)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491593
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3434 | IFIT1 | interferon induced protein ... | NM_001548.5 | 99.8% | 100% | 465A>G;525T>G |
| 2 | human | 3434 | IFIT1 | interferon induced protein ... | NM_001270927.2 | 99.7% | 99.7% | 4T>A;465A>G;525T>G |
| 3 | human | 3434 | IFIT1 | interferon induced protein ... | NM_001270928.2 | 93.3% | 93.5% | 0_1ins93;372A>G;432T>G |
| 4 | human | 3434 | IFIT1 | interferon induced protein ... | NM_001270929.2 | 93.3% | 93.5% | 0_1ins93;372A>G;432T>G |
| 5 | human | 3434 | IFIT1 | interferon induced protein ... | NM_001270930.1 | 93.3% | 93.5% | 0_1ins93;372A>G;432T>G |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1500
- ORF length:
- 1434
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag tacaaatggt gatgatcatc aggtcaagga tagtctggag caattgagat 121 gtcactttac atgggagtta tccattgatg acgatgaaat gcctgattta gaaaacagag 181 tcttggatca gattgaattc ctagacacca aatacagtgt gggaatacac aacctactag 241 cctatgtgaa acacctgaaa ggccagaatg aggaagccct gaagagctta aaagaagctg 301 aaaacttaat gcaggaagaa catgacaacc aagcaaatgt gaggagtctg gtgacctggg 361 gcaactttgc ctggatgtat taccacatgg gcagactggc agaagcccag acttacctgg 421 acaaggtgga gaacatttgc aagaagcttt caaatccctt ccgctataga atggagtgtc 481 cagaaataga ctgtgaggaa ggatgggcct tgctgaagtg tggaggaaag aattatgaac 541 gggccaaggc ctgctttgaa aaggtgcttg aagtggaccc tgaaaacccg gaatccagcg 601 ctgggtatgc gatctctgcc tatcgcctgg atggctttaa attagccaca aaaaatcaca 661 agccattttc tttgcttccc ctaaggcagg ctgtccgctt aaatccagac aatggatata 721 ttaaggttct ccttgccctg aagcttcagg atgaaggaca ggaagctgaa ggagaaaagt 781 acattgaaga agctctagcc aacatgtcct cacagaccta tgtctttcga tatgcagcca 841 agttttaccg aagaaaaggc tctgtggata aagctcttga gttattaaaa aaggccttgc 901 aggaaacacc cacttctgtc ttactgcatc accagatagg gctttgctac aaggcacaaa 961 tgatccaaat caaggaggct acaaaagggc agcctagagg gcagaacaga gaaaagctag 1021 acaaaatgat aagatcagcc atatttcatt ttgaatctgc agtggaaaaa aagcccacat 1081 ttgaggtggc TCATCTAGAC CTGGCAAGAA TGTATATAGA AGCAGGCAAT CACAGAAAAG 1141 CTGAAGAGAA TTTTCAAAAA TTGTTATGCA TGAAACCAGT GGTAGAAGAA ACAATGCAAG 1201 ACATACATTT CCACTATGGT CGGTTTCAGG AATTTCAAAA GAAATCTGAC GTCAATGCAA 1261 TTATCCATTA TTTAAAAGCT ATAAAAATAG AACAGGCATC ATTAACAAGG GATAAAAGTA 1321 TCAATTCTTT GAAGAAATTG GTTTTAAGGA AACTTCGGAG AAAGGCATTA GATCTGGAAA 1381 GCTTGAGCCT CCTTGGGTTC GTCTACAAAT TGGAAGGAAA TATGAATGAA GCCCTGGAGT 1441 ACTATGAGCG GGCCCTGAGA CTGGCTGCTG ACTTTGAGAA CTCTGTGAGA CAAGGTCCTT 1501 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1561 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1621 TTTATATATC TTGTGGAAAG GACGAGCTTC TTGCCTGACC TTGTTTTGAA CGCGTTAAGT 1681 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt