Construct: ORF TRCN0000491595
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008566.2_s317c1
- Derived from:
- ccsbBroadEn_00323
- DNA Barcode:
- AGCTTAGACTTGATGCCTCACACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CLU (1191)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491595
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1191 | CLU | clusterin | NM_001831.4 | 100% | 100% | |
| 2 | human | 1191 | CLU | clusterin | XM_006716284.3 | 89.6% | 89.6% | 1_156del |
| 3 | human | 1191 | CLU | clusterin | NR_045494.1 | 46.9% | 1_180del;1528_2871del | |
| 4 | human | 1191 | CLU | clusterin | NR_038335.1 | 44.7% | 1_321del;1669_3012del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1413
- ORF length:
- 1347
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat gaagactctg ctgctgtttg tggggctgct gctgacctgg gagagtgggc 121 aggtcctggg ggaccagacg gtctcagaca atgagctcca ggaaatgtcc aatcagggaa 181 gtaagtacgt caataaggaa attcaaaatg ctgtcaacgg ggtgaaacag ataaagactc 241 tcatagaaaa aacaaacgaa gagcgcaaga cactgctcag caacctagaa gaagccaaga 301 agaagaaaga ggatgcccta aatgagacca gggaatcaga gacaaagctg aaggagctcc 361 caggagtgtg caatgagacc atgatggccc tctgggaaga gtgtaagccc tgcctgaaac 421 agacctgcat gaagttctac gcacgcgtct gcagaagtgg ctcaggcctg gttggccgcc 481 agcttgagga gttcctgaac cagagctcgc ccttctactt ctggatgaat ggtgaccgca 541 tcgactccct gctggagaac gaccggcagc agacgcacat gctggatgtc atgcaggacc 601 acttcagccg cgcgtccagc atcatagacg agctcttcca ggacaggttc ttcacccggg 661 agccccagga tacctaccac tacctgccct tcagcctgcc ccaccggagg cctcacttct 721 tctttcccaa gtcccgcatc gtccgcagct tgatgccctt ctctccgtac gagcccctga 781 acttccacgc catgttccag cccttccttg agatgataca cgaggctcag caggccatgg 841 acatccactt ccatagcccg gccttccagc acccgccaac agaattcata cgagaaggcg 901 acgatgaccg gactgtgtgc cgggagatcc gccacaactc cacgggctgc ctgcggatga 961 aggaccagtg tgacaagtgc cgggagatct tgtctgtgga ctgttccacc aacaacccct 1021 cccaggctaa gctgcggcgg gagctcgacg aatccctcca ggtcgctgag aggttGACCA 1081 GGAAATACAA CGAGCTGCTA AAGTCCTACC AGTGGAAGAT GCTCAACACC TCCTCCTTGC 1141 TGGAGCAGCT GAACGAGCAG TTTAACTGGG TGTCCCGGCT GGCAAACCTC ACGCAAGGCG 1201 AAGACCAGTA CTATCTGCGG GTCACCACGG TGGCTTCCCA CACTTCTGAC TCGGACGTTC 1261 CTTCCGGTGT CACTGAGGTG GTCGTGAAGC TCTTTGACTC TGATCCCATC ACTGTGACGG 1321 TCCCTGTAGA AGTCTCCAGG AAGAACCCTA AATTTATGGA GACCGTGGCG GAGAAAGCGC 1381 TGCAGGAATA CCGCAAAAAG CACCGGGAGG AGTGCCCAAC TTTCTTGTAC AAAGTGGTTG 1441 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1501 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAG 1561 CTTAGACTTG ATGCCTCACA CCACGCGTTA AGTCgacaat caacctctgg attacaaaat 1621 ttgtgaaaga tt