Construct: ORF TRCN0000491608
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001831.1_s317c1
- Derived from:
- ccsbBroadEn_13534
- DNA Barcode:
- TGAATCCGTGCCATCCGGATAATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RUSC1-AS1 (284618)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491608
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 284618 | RUSC1-AS1 | RUSC1 antisense RNA 1 | NR_145426.1 | 22% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 444
- ORF length:
- 375
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagccggga gggtcagaga acgcggctgc gctctggatc TCCGAAGGGG 121 GGCGGGGGCC TGGAAGAGGT CCGGGCCCAG AATGGACCTC CAGGTCCCTG CTGCCGCAAT 181 CTGGCCCTGC CCTCCAGCCT ACCCCTTACT CCCAGAGGAA GGGACCCAGG GAGACCCACC 241 CAGATGCCCT GAAAGGAGGT GGAGGATGGG GGTGGGGCAA TACGCAGAGT TTGTCTGGTG 301 AATGCAGGAA AGGGGTGGGG GCTGGAGAGG AAAAGGATGG AGCAGCCGTC AGCTTGTCAA 361 CTCCGCATCT GCTGGCTGCC TCGGCCGGGC TCCAGCCTGC GCCCTCGCCC CTCGGAACAG 421 CGGCACGCTG CAATCCTGTA CCCTTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 481 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 541 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT GAATCCGTGC 601 CATCCGGATA ATTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 661 att