Construct: ORF TRCN0000491609
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016140.2_s317c1
- Derived from:
- ccsbBroadEn_15228
- DNA Barcode:
- ACCGTTTCGCCACGCAATTAGTAT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- BATF2 (116071)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491609
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 116071 | BATF2 | basic leucine zipper ATF-li... | NM_001300808.1 | 99.4% | 6.8% | 18_19insN;21_22delAGinsNN |
| 2 | human | 116071 | BATF2 | basic leucine zipper ATF-li... | NM_001300807.1 | 75.2% | 5.2% | 1_183del;201_202insN;204_205delAGinsNN |
| 3 | human | 116071 | BATF2 | basic leucine zipper ATF-li... | NM_138456.4 | 68.6% | 4.7% | 1_255del;273_274insN;276_277delAGinsNN |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 120
- ORF length:
- 54
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ttgtgcctcc tgcntcnnct ccagggctcc tgggctgctg ggaccaggct 121 gaggggctcc tgggccctgg cccacaggga caacatggct gccgggagca gctggagctg 181 ttccagaccc cgggttcctg ttacccagct cagccgctct ctccaggtcc acagcctcat 241 gattctccca gcctcctcca gtgccccctg ccctcactgt cccttggccc cgctgtggtt 301 gctgaaccTC CTGTCCAGCT GTCCCCCAGC CCTCTCCTGT TTGCCTCGCA CACTGGTTCC 361 AGCCTGCAGG GGTCTTCCTC TAAGCTCAGT GCCCTCCAGC CCAGCCTCAC GGCCCAAACT 421 GCCCCTCCAC AGCCCCTCGA GCTGGAGCAT CCCACCAGAG GGAAGCTGGG GTCCTCTCCC 481 GACAACCCTT CCTCTGCCCT GGGGCTTGCA CGTCTGCAGA GCAGGGAGCA CAAACCTGCT 541 CTCTCAGCAG CCACTTGGCA AGGGCTGGTT GTGGATCCCA GCCCTCACCC TCTCCTGGCC 601 TTTCCTCTGC TCTCCTCTGC TCAAGTCCAC TTCTACCCAA CTTTCTTGTA CAAAGTGGTT 661 GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT 721 AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA 781 CCGTTTCGCC ACGCAATTAG TATACGCGTT AAGTCgacaa tcaacctctg gattacaaaa 841 tttgtgaaag att