Construct: ORF TRCN0000491614
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020654.2_s317c1
- DNA Barcode:
- CTAGCTGAGTGTCGGCTGGCCGCT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PDK1 (5163)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491614
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5163 | PDK1 | pyruvate dehydrogenase kina... | NM_002610.5 | 100% | 100% | |
| 2 | human | 5163 | PDK1 | pyruvate dehydrogenase kina... | NM_001278549.2 | 95.6% | 95.3% | 411_470del |
| 3 | human | 5163 | PDK1 | pyruvate dehydrogenase kina... | XM_011511345.3 | 82.5% | 82.5% | 0_1ins228 |
| 4 | human | 5163 | PDK1 | pyruvate dehydrogenase kina... | XM_011511343.2 | 78.9% | 78.7% | 0_1ins228;183_242del |
| 5 | human | 5163 | PDK1 | pyruvate dehydrogenase kina... | XM_011511344.2 | 78.9% | 78.7% | 0_1ins228;183_242del |
| 6 | human | 5163 | PDK1 | pyruvate dehydrogenase kina... | XM_011511347.2 | 78.9% | 78.7% | 0_1ins228;183_242del |
| 7 | human | 5163 | PDK1 | pyruvate dehydrogenase kina... | XR_427094.1 | 78.1% | (many diffs) | |
| 8 | human | 5163 | PDK1 | pyruvate dehydrogenase kina... | XR_922943.2 | 77.5% | 1_61del;472_531del;1430_1686del | |
| 9 | human | 5163 | PDK1 | pyruvate dehydrogenase kina... | XR_001738772.2 | 75.6% | 1_61del;472_531del;1430_1729del | |
| 10 | human | 5163 | PDK1 | pyruvate dehydrogenase kina... | XR_001738775.2 | 74.4% | 1_73del;1382_1758del | |
| 11 | human | 5163 | PDK1 | pyruvate dehydrogenase kina... | XR_922945.1 | 72.4% | 1_61del;472_531del;1430_1806del | |
| 12 | human | 5163 | PDK1 | pyruvate dehydrogenase kina... | XR_001738773.1 | 71.9% | 1_61del;472_531del;1430_1819del | |
| 13 | human | 5163 | PDK1 | pyruvate dehydrogenase kina... | XR_922942.2 | 69.6% | 1_61del;472_531del;1430_1877del | |
| 14 | human | 5163 | PDK1 | pyruvate dehydrogenase kina... | XM_006712594.2 | 56.8% | 55.7% | (many diffs) |
| 15 | human | 5163 | PDK1 | pyruvate dehydrogenase kina... | XM_006712595.3 | 55.6% | 54.8% | (many diffs) |
| 16 | human | 5163 | PDK1 | pyruvate dehydrogenase kina... | NR_103731.1 | 54.5% | (many diffs) | |
| 17 | human | 5163 | PDK1 | pyruvate dehydrogenase kina... | NR_103729.2 | 44.3% | 1_61del;1370_2952del | |
| 18 | human | 5163 | PDK1 | pyruvate dehydrogenase kina... | XR_427093.3 | 43.4% | 1_61del;472_531del;1430_3008del | |
| 19 | human | 5163 | PDK1 | pyruvate dehydrogenase kina... | XR_001738776.1 | 40.9% | (many diffs) | |
| 20 | human | 5163 | PDK1 | pyruvate dehydrogenase kina... | XR_001738774.2 | 39.1% | 1_73del;1382_3337del | |
| 21 | human | 5163 | PDK1 | pyruvate dehydrogenase kina... | XR_922944.2 | 38.6% | 1_61del;472_531del;1430_3385del | |
| 22 | human | 5163 | PDK1 | pyruvate dehydrogenase kina... | XR_001738771.1 | 36.1% | 1_61del;472_531del;1430_3619del | |
| 23 | human | 5163 | PDK1 | pyruvate dehydrogenase kina... | XR_427095.3 | 28.9% | 1_73del;841_842ins77;1305_4168del | |
| 24 | mouse | 228026 | Pdk1 | pyruvate dehydrogenase kina... | NM_172665.5 | 85.6% | 91.5% | (many diffs) |
| 25 | mouse | 228026 | Pdk1 | pyruvate dehydrogenase kina... | XM_011239444.1 | 79% | 84.3% | (many diffs) |
| 26 | mouse | 228026 | Pdk1 | pyruvate dehydrogenase kina... | XM_017317590.1 | 72.4% | 78.6% | (many diffs) |
| 27 | mouse | 228026 | Pdk1 | pyruvate dehydrogenase kina... | XM_011239445.2 | 66.8% | 72.5% | (many diffs) |
| 28 | mouse | 228026 | Pdk1 | pyruvate dehydrogenase kina... | XM_011239446.2 | 66.8% | 72.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 102
- ORF end:
- 1410
- ORF length:
- 1308
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggctccgc ggccgccccc ttcaccgaat tcggactcgg catgaggctg gcgcggctgc 121 ttcgcggagc cgccttggcc ggcccgggcc cggggctgcg cgccgccggc ttcagccgca 181 gcttcagctc ggactcgggc tccagcccgg cgtccgagcg cggcgttccg ggccaggtgg 241 acttctacgc gcgcttctcg ccgtccccgc tctccatgaa gcagttcctg gacttcggat 301 cagtgaatgc ttgtgaaaag acctcattta tgtttctgcg gcaagagttg cctgtcagac 361 tggcaaatat aatgaaagaa ataagtctcc ttccagataa tcttctcagg acaccatccg 421 ttcaattggt acaaagctgg tatatccaga gtcttcagga gcttcttgat tttaaggaca 481 aaagtgctga ggatgctaaa gctatttatg actttacaga tactgtgata cggatcagaa 541 accgacacaa tgatgtcatt cccacaatgg cccagggtgt gattgaatac aaggagagct 601 ttggggtgga tcctgtcacc agccagaatg ttcagtactt tttggatcga ttctacatga 661 gtcgcatttc aattagaatg ttactcaatc agcactcttt attgtttggt ggaaaaggca 721 aaggaagtcc atctcatcga aaacacattg gaagcataaa tccaaactgc aatgtacttg 781 aagttattaa agatggctat gaaaatgcta ggcgtctgtg tgatttgtat tatattaact 841 ctcccgaact agaacttgaa gaactaaatg caaaatcacc aggacagcca atacaagtgg 901 tttatgtacc atcccatctc tatcacatgg tgtttgaact tttcaagaat gcaatgagag 961 ccactatgga acaccatgcc aacAGAGGTG TTTACCCCCC TATTCAAGTT CATGTCACGC 1021 TGGGTAATGA GGATTTGACT GTGAAGATGA GTGACCGAGG AGGTGGCGTT CCTTTGAGGA 1081 AAATTGACAG ACTTTTCAAC TACATGTATT CAACTGCACC AAGACCTCGT GTTGAGACCT 1141 CCCGCGCAGT GCCTCTGGCT GGTTTTGGTT ATGGATTGCC CATATCACGT CTTTACGCAC 1201 AATACTTCCA AGGAGACCTG AAGCTGTATT CCCTAGAGGG TTACGGGACA GATGCAGTTA 1261 TCTACATTAA GGCTCTGTCA ACAGACTCAA TAGAAAGACT CCCAGTGTAT AACAAAGCTG 1321 CCTGGAAGCA TTACAACACC AACCACGAGG CTGATGACTG GTGCGTCCCC AGCAGAGAAC 1381 CCAAAGACAT GACGACGTTC CGCAGTGCCT AGAAGCTTAA GGGTGGGCGC GCCGACCCAG 1441 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1501 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1561 TATCTTGTGG AAAGGACGAC TAGCTGAGTG TCGGCTGGCC GCTACGCGTT AAGTCgacaa 1621 tcaacctctg gattacaaaa tttgtgaaag att