Construct: ORF TRCN0000491632
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021001.2_s317c1
- DNA Barcode:
- TTTTAAGCCAAGGCCATCCTTAGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- DRD2 (1813)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491632
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1813 | DRD2 | dopamine receptor D2 | NM_016574.3 | 100% | 100% | |
| 2 | human | 1813 | DRD2 | dopamine receptor D2 | NM_000795.4 | 93.4% | 93.4% | 723_809del |
| 3 | human | 1813 | DRD2 | dopamine receptor D2 | XM_017017296.2 | 93.4% | 93.4% | 723_809del |
| 4 | mouse | 13489 | Drd2 | dopamine receptor D2 | XM_006509996.3 | 90.2% | 95.4% | (many diffs) |
| 5 | mouse | 13489 | Drd2 | dopamine receptor D2 | NM_010077.2 | 84.3% | 89.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1314
- ORF length:
- 1242
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggatcca ctgaatctgt cctggtatga tgatgatctg gagaggcaga 121 actggagccg gcccttcaac gggtcagacg ggaaggcgga cagaccccac tacaactact 181 atgccacact gctcaccctg ctcatcgctg tcatcgtctt cggcaacgtg ctggtgtgca 241 tggctgtgtc ccgcgagaag gcgctgcaga ccaccaccaa ctacctgatc gtcagcctcg 301 cagtggccga cctcctcgtc gccacactgg tcatgccctg ggttgtctac ctggaggtgg 361 taggtgagtg gaaattcagc aggattcact gtgacatctt cgtcactctg gacgtcatga 421 tgtgcacggc gagcatcctg aacttgtgtg ccatcagcat cgacaggtac acagctgtgg 481 ccatgcccat gctgtacaat acgcgctaca gctccaagcg ccgggtcacc gtcatgatct 541 ccatcgtctg ggtcctgtcc ttcaccatct cctgcccact cctcttcgga ctcaataacg 601 cagaccagaa cgagtgcatc attgccaacc cggccttcgt ggtctactcc tccatcgtct 661 ccttctacgt gcccttcatt gtcaccctgc tggtctacat caagatctac attgtcctcc 721 gcagacgccg caagcgagtc aacaccaaac gcagcagccg agctttcagg gcccacctga 781 gggctccact aaaggaggct gcccggcgag cccaggagct ggagatggag atgctctcca 841 gcaccagccc acccgagagg acccggtaca gccccatccc acccagccac caccagctga 901 ctctccccga cccgtcccac catggtcTCC ACAGCACTCC CGACAGCCCC GCCAAACCAG 961 AGAAGAATGG GCATGCCAAA GACCACCCCA AGATTGCCAA GATCTTTGAG ATCCAGACCA 1021 TGCCCAATGG CAAAACCCGG ACCTCCCTCA AGACCATGAG CCGTAGGAAG CTCTCCCAGC 1081 AGAAGGAGAA GAAAGCCACT CAGATGCTCG CCATTGTTCT CGGCGTGTTC ATCATCTGCT 1141 GGCTGCCCTT CTTCATCACA CACATCCTGA ACATACACTG TGACTGCAAC ATCCCGCCTG 1201 TCCTGTACAG CGCCTTCACG TGGCTGGGCT ATGTCAACAG CGCCGTGAAC CCCATCATCT 1261 ACACCACCTT CAACATTGAG TTCCGCAAGG CCTTCCTGAA GATCCTCCAC TGCTGAGACC 1321 CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1381 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1441 ATATATCTTG TGGAAAGGAC GATTTTAAGC CAAGGCCATC CTTAGTACGC GTTAAGTCga 1501 caatcaacct ctggattaca aaatttgtga aagatt