Construct: ORF TRCN0000491643
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012066.1_s317c1
- Derived from:
- ccsbBroadEn_09585
- DNA Barcode:
- ACAAGGCCCATCTGCCTTGGAATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ASB6 (140459)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491643
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 140459 | ASB6 | ankyrin repeat and SOCS box... | NM_177999.3 | 99.8% | 100% | 207G>C |
| 2 | human | 140459 | ASB6 | ankyrin repeat and SOCS box... | NM_017873.4 | 46.7% | 32.3% | 207G>C;402_510del;701_1263del |
| 3 | human | 140459 | ASB6 | ankyrin repeat and SOCS box... | NM_001202403.1 | 46.4% | 36% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 657
- ORF length:
- 591
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc gttcctgcac ggcttccgga ggatcatctt cgagtaccag ccgctggtgg 121 atgcgattct gggctccctg gggatccagg accccgagcg gcaggagtct ctggaccggc 181 ccagttatgt cgccagcgag gagagccgaa tccttgttct cactgagctg ctggagagga 241 aagcccactc tcccttttac caggaaggcg tcagcaacgc cctgctcaag atggctgagc 301 tggggctgac gcgggcggcc gacgttcTCT TGCGGCATGG GGCCAATCTC AACTTTGAAG 361 ACCCAGTCAC CTACTACACG GCCTTGCACA TCGCCGTCCT GCGGAACCAG CCGGACATGG 421 TGGAGCTGCT GGTGCATCAC GGGGCCGACG TTAATCGGAG GGACCGGGAA AAACTGCTCT 481 GCTCCATGCT CTGGCCAGCA GCGACGGGGT GCAGATCCAC AATACTGAGA ACATTCGTCT 541 CTTACTGGAA GGAGGGGCAG ACGTCAAGGC CACCACCAAA GATGGGGACA CAGTGTTCAC 601 CTGCATCATC TTCCTGCTTG GTGAGACCGT GGGAGGGGAC AAAGAGGAGG CCCAGATGCC 661 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 721 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 781 ATATATCTTG TGGAAAGGAC GAACAAGGCC CATCTGCCTT GGAATAACGC GTTAAGTCga 841 caatcaacct ctggattaca aaatttgtga aagatt