Construct: ORF TRCN0000491645
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009798.1_s317c1
- Derived from:
- ccsbBroadEn_10444
- DNA Barcode:
- TCCCGACTAGAATAAACCTGTGTT
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SEC14L1 (6397)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491645
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6397 | SEC14L1 | SEC14 like lipid binding 1 | NM_001204410.1 | 99.7% | 99.7% | (many diffs) |
| 2 | human | 6397 | SEC14L1 | SEC14 like lipid binding 1 | NM_001143998.2 | 99.7% | 99.5% | (many diffs) |
| 3 | human | 6397 | SEC14L1 | SEC14 like lipid binding 1 | NM_001143999.2 | 99.7% | 99.5% | (many diffs) |
| 4 | human | 6397 | SEC14L1 | SEC14 like lipid binding 1 | NM_003003.4 | 99.7% | 99.5% | (many diffs) |
| 5 | human | 6397 | SEC14L1 | SEC14 like lipid binding 1 | NM_001039573.3 | 99% | 99% | (many diffs) |
| 6 | human | 6397 | SEC14L1 | SEC14 like lipid binding 1 | NM_001204408.2 | 99% | 99% | (many diffs) |
| 7 | human | 6397 | SEC14L1 | SEC14 like lipid binding 1 | NM_001144001.2 | 94.9% | 94.8% | (many diffs) |
| 8 | human | 729799 | SEC14L1P1 | SEC14 like 1 pseudogene 1 | NR_026952.1 | 73.9% | (many diffs) | |
| 9 | mouse | 74136 | Sec14l1 | SEC14-like lipid binding 1 | NM_001166506.1 | 86% | 94.1% | (many diffs) |
| 10 | mouse | 74136 | Sec14l1 | SEC14-like lipid binding 1 | NM_001166507.1 | 85.8% | 94% | (many diffs) |
| 11 | mouse | 74136 | Sec14l1 | SEC14-like lipid binding 1 | NM_028777.3 | 85.5% | 93.6% | (many diffs) |
| 12 | mouse | 74136 | Sec14l1 | SEC14-like lipid binding 1 | XM_017314804.1 | 81.8% | 89.5% | (many diffs) |
| 13 | mouse | 74136 | Sec14l1 | SEC14-like lipid binding 1 | XM_006534352.1 | 81.2% | 89% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2214
- ORF length:
- 2145
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggtgcagaaa taccagtccc cagtgagggt gtacaaatac ccctttgaat 121 taattatggc tgcctatgaa aggaggttcc ctacatgtcc tttgattccg atgttcgtgg 181 gcagtgacac tgtgaatgaa ttcaagagcg aagatggggc tattcatgtc attgaaaggc 241 gctgcaagtt ggatgtagat gcacccagac tgctgaagaa gattgcagga gttgattatg 301 tttattttgt ccagaaaaac tcactgaatt ctcgggaacg tactttgcac attgaggctt 361 ataatgaaac gttttccaat cgggtcatca ttaatgagca ttgctgctac accgttcacc 421 ctgaaaatga agattggacc tgttttgaac agtctgcaag tttagatatt aaatctttct 481 ttggttttga aagtacagtg gaaaaaattg caatgaaaca atataccagc aacattaaaa 541 aaggaaagga aatcatcgaa tactaccttc gccaattaga agaagaaggc ataacctttg 601 tgccccgttg gagtccgcct tccatcacgc cctcttcaga gacatcttca tcatcctcca 661 agaaacaagc agcgtccatg gccgtcgtca tcccagaagc tgccctcaag gaggggctga 721 gtggtgatgc cctcagcagc cccagtgcac ctgagcccgt ggtgggcacc cctgacgaca 781 aactagatgc cgactacatc aagagatacc tgggcgattt gactccgctg caggagagct 841 gcctcattag acttcgccag tggctccagg agacccacaa gggcaaaatt ccaaaagatg 901 agcatattct tcggttcctc cgtgcacggg attttaatat tgacaaagcc agagagatca 961 tgtgtcagtc tttgacgtgg agaaagcagc atcaggtaga ctacattctt gaaacctgga 1021 cccctcctca ggtccttcag gattactacg cgggaggctg gcatcatcac gacaaagatg 1081 ggcggcccct ctacgtgctc aggctagggc agatggacac caaaggcttg gtgagagcgc 1141 tcggggagga agccctgctg agatacgttc tctccataaa tgaagaaggg ctaaggcgat 1201 gcgaagagaa tacaaaagtc tttggtcggc ctatcagctc atggacctgc ctggtggact 1261 tggaagggct gaacatgcgc cacttgtgga gacctggtgt gaaagcgctg ctgcggatca 1321 tggaggtggt ggaggccaac taccctgaga cactgggccg ccttctcatc ctgcgggcgc 1381 ccagggtatt tcctgtgctc tggacgctgg ttagtccgtt cattgatgac aacaccagaa 1441 ggaagttcct catttatgca ggaaatgact accagggtcc tggaggcctg ctggattaca 1501 tcgacaaaga gattattcca gatttcctga gtggggagtg catgtgcgaa gtgccagagg 1561 gtggactggt ccccaaatct ctgtaccgga ctgcagagga gctggagaac gaagacctga 1621 agctctggac tgagaccatc taccagtctg caagcgtctt caaaggagcc ccacatgaga 1681 ttctcattca gattgtggat gcctcgtcag tcatcacttg ggatttcgac gtgtgcaaag 1741 gggacattgt gtttaacatc tatcactcca agaggtcgcc acaaccaccc aaaaaggact 1801 ccctgggagc ccacagcaTC ACCTCTCCGG GTGGGAACAA TGTGCAGCTC ATAGACAAAG 1861 TCTGGCAGCT GGGCCGCGAC TACAGCATGG TGGAGTCGCC TCTGATCTGC AAAGAAGGAG 1921 AAAGCGTGCA GGGTTCCCAT GTGACCAGGT GGCCGGGCTT CTACATCCTG CAGTGGAAAT 1981 TCCACAGCAT GCCTGCGTGC GCCGCCAGCA GCCTTCCCCG GGTGGACGAC GTGCTTGCGT 2041 CCCTGCAGGT CTCTTCGCAC AAGTGTAAAG TGATGTACTA CACCGAGGTG ATCGGCTCGG 2101 AGGATTTCAG AGGTTCCATG ACGAGCCTGG AGTCCAGCCA CAGCGGCTTC TCCCAGCTGA 2161 GTGCCGCCAC CACCTCCTCC AGCCAGTCCC ACTCCAGCTC CATGATCTCA GGTTGCCAAC 2221 TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA 2281 TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT 2341 ATCTTGTGGA AAGGACGATC CCGACTAGAA TAAACCTGTG TTACGCGTTA AGTCgacaat 2401 caacctctgg attacaaaat ttgtgaaaga tt