Construct: ORF TRCN0000491648
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020639.2_s317c1
- DNA Barcode:
- CATACTACTGGGATGTCGTAAAGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- SIK2 (23235)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491648
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23235 | SIK2 | salt inducible kinase 2 | NM_015191.3 | 100% | 100% | |
| 2 | human | 23235 | SIK2 | salt inducible kinase 2 | XM_017017417.1 | 92.4% | 92.4% | 0_1ins210 |
| 3 | human | 23235 | SIK2 | salt inducible kinase 2 | XM_017017418.1 | 83.4% | 83.4% | 0_1ins459 |
| 4 | mouse | 235344 | Sik2 | salt inducible kinase 2 | NM_178710.3 | 88.3% | 90.7% | (many diffs) |
| 5 | mouse | 235344 | Sik2 | salt inducible kinase 2 | XM_006510266.1 | 88.3% | 90.8% | (many diffs) |
| 6 | mouse | 235344 | Sik2 | salt inducible kinase 2 | XM_006510267.2 | 85.9% | 88.6% | (many diffs) |
| 7 | mouse | 235344 | Sik2 | salt inducible kinase 2 | XM_017313332.1 | 85.9% | 88.6% | (many diffs) |
| 8 | mouse | 235344 | Sik2 | salt inducible kinase 2 | XR_379116.1 | 36.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2850
- ORF length:
- 2778
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggtcatg gcggatggcc cgaggcactt gcagcgcggg ccggtccggg 121 tggggttcta cgacatcgag ggcacgctgg gcaagggcaa cttcgctgtg gtgaagctgg 181 ggcggcaccg gatcaccaag acggaggtgg caataaaaat aatcgataag tctcagctgg 241 atgcagtgaa ccttgagaaa atctaccgag aagtacaaat aatgaaaatg ttagaccacc 301 ctcacataat caaactttat caggtaatgg agaccaaaag tatgttgtac cttgtgacag 361 aatatgccaa aaatggagaa atttttgact atcttgctaa tcatggccgg ttaaatgagt 421 ctgaagccag gcgaaaattc tggcaaatcc tgtctgctgt tgattattgt catggtcgga 481 agattgtgca ccgtgacctc aaagctgaaa atctcctgct ggataacaac atgaatatca 541 aaatagcaga tttcggtttt ggaaatttct ttaaaagtgg tgaactgctg gcaacatggt 601 gtggcagccc cccttatgca gccccagaag tctttgaagg gcagcagtat gaaggaccac 661 agctggacat ctggagtatg ggagttgttc tttatgtcct tgtctgtgga gctctgccct 721 ttgatggacc gactcttcca attttgaggc agagggttct ggaaggaaga ttccggattc 781 cgtatttcat gtcagaagat tgcgagcacc ttatccgaag gatgttggtc ctagacccat 841 ccaaacggct aaccatagcc caaatcaagg agcataaatg gatgctcata gaagttcctg 901 tccagagacc tgttctctat ccacaagagc aagaaaatga gccatccatc ggggagttta 961 atgagcaggt tctgcgactg atgcacagcc ttggaataga tcagcagaaa accattgagt 1021 ctttgcagaa caagagctat aaccactttg ctgccattta tttcttgttg gtggagcgcc 1081 tgaaatcaca tcggagcagt ttcccagtgg agcagagact tgatggccgc cagcgtcggc 1141 ctagcaccat tgctgagcaa acagttgcca aggcacagac tgtggggctc ccagtgacca 1201 tgcattcacc gaacatgagg ctgctgcgat ctgccctcct cccccaggca tccaacgtgg 1261 aggccttttc atttccagca tctggctgtc aggcggaagc tgcattcatg gaagaagagt 1321 gtgtggacac tccaaaggtc aatggctgtc tgcttgaccc tgtgcctcct gtcctggtgc 1381 ggaagggatg ccagtcactg cccagcaaca tgatggagac ctccattgac gaagggctgg 1441 agacagaagg agaggccgag gaagaccccg ctcatgcctt tgaggcattt cagtccacac 1501 gcagcgggca gagacggcac actctgtcag aagtgaccaa tcaactggtc gtgatgcctg 1561 gggcagggaa aattttctcc atgaatgaca gcccctccct tgacagtgtg gactctgagt 1621 atgatatggg gtctgttcag agggacctga actttctgga agacaaccct tcccttaagg 1681 acatcatgtt agccaatcag ccttcacccc gcatgacatc tcccttcata agcctgagac 1741 ctaccaaccc agccatgcag gctctgagct cccagaaacg agaggtccac aacaggtctc 1801 cagtgagctt cagagagggc cgcagagcat cagatacctc cctcacccag ggaattgtag 1861 catttagaca acatcttcag aatctggcta gaaccaaagg aattctagag ttgaacaaag 1921 tgcagttgtt gtatgaacaa ataggaccgg aggcagaccc taacctggcg ccggcggctc 1981 ctcagctcca ggaccttgct agcagctgcc ctcaggaaga agtttctcag cagcaggaaa 2041 gcgtctccac tctccctgcc agcgtgcatc cccagctgtc cccacggcag agcctggaga 2101 cccagtacct gcagcacaga ctccagaagc ccagccttct gtcaaaggcc cagaacacct 2161 gtcagcttta ttgcaaagaa ccaccgcgga gccttgagca gcagctgcag gaacataggc 2221 tccagcagaa gcgactcttt cttcagaagc agtctcaact gcaggcctat tttaatcaga 2281 tgcagatagc agagagctcc tacccacagc caagtcagca gctgcccctt ccccgccagg 2341 agactccacc gccttctcag caggccccac cgttcagcct gacccagccc ctgagccccg 2401 tcctggagcc ttcctccgag cagatgcaat acagcccttt cctcagccag taccaagaga 2461 tgcagcttca gcccctgccc tccacttccg gtccccgggc tgctcctcct ctgcccacgc 2521 agctacagca gcagcagccg ccaccgccac cacccccTCC ACCACCACGA CAGCCAGGAG 2581 CTGCCCCAGC CCCCTTACAG TTCTCCTATC AGACTTGTGA GCTGCCAAGC GCTGCTTCCC 2641 CTGCGCCAGA CTATCCCACT CCCTGTCAGT ATCCTGTGGA TGGAGCCCAG CAGAGCGACC 2701 TAACGGGGCC AGACTGTCCC AGAAGCCCAG GACTGCAAGA GGCCCCCTCC AGCTACGACC 2761 CACTAGCCCT CTCTGAGCTA CCTGGACTCT TTGATTGTGA AATGCTAGAC GCTGTGGATC 2821 CACAACACAA CGGGTATGTC CTGGTGAATT AGGACCCAGC TTTCTTGTAC AAAGTGGTTG 2881 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 2941 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACA 3001 TACTACTGGG ATGTCGTAAA GTACGCGTTA AGTCgacaat caacctctgg attacaaaat 3061 ttgtgaaaga tt