Construct: ORF TRCN0000491657
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004346.1_s317c1
- Derived from:
- ccsbBroadEn_02006
- DNA Barcode:
- GGGTGGCCTTCCAATCGAATCCGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ADAM18 (8749)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491657
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8749 | ADAM18 | ADAM metallopeptidase domai... | NM_001190956.2 | 100% | 100% | |
| 2 | human | 8749 | ADAM18 | ADAM metallopeptidase domai... | NM_001320313.2 | 25.1% | 24.4% | (many diffs) |
| 3 | human | 8749 | ADAM18 | ADAM metallopeptidase domai... | NM_014237.3 | 24.3% | 23.6% | (many diffs) |
| 4 | human | 8749 | ADAM18 | ADAM metallopeptidase domai... | NR_135201.2 | 23.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 612
- ORF length:
- 546
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt ccttctcctc gccctcctca ctgagcttgg aagactgcaa gcccacgaag 121 gttctgaagg aatatttctg catgtcacag ttccacggaa gattaagtca aatgacagtg 181 aagtttcaga gaggaagatg atttacatca ttacaattga tggacaacct tacactctac 241 atctcGGAAA ACAATCATTC TTACCCCAGA ACTTTTTGGT TTATACATAT AATGAAACTG 301 GATCTTTGCA TTCTGTGTCT CCATATTTTA TGATGCATTG CCATTACCAA GGATATGCTG 361 CCGAATTTCC AAATTCATTT GTGACACTCA GTATATGTTC TGGTCTCAGG GGATTTCTCC 421 AGTTTGAAAA TATCAGTTAT GGAATTGAAC CAGTAGAATC TTCAGCAAGA TTTGAGCATA 481 TAATTTATCA AATGAAAAAT AATGATCCAA ATGTATCCAT TTTAGCAGTA AATTACAGTC 541 ATATTTGGCA GAAAGACCAG CCCTACAAAG TTCCTTTAAA CTCACAGGTG ACTGTCATCA 601 TTCTGATGTT ATGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 661 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 721 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAGGG TGGCCTTCCA ATCGAATCCG 781 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t