Construct: ORF TRCN0000491689

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020636.2_s317c1
DNA Barcode:
AGGCTTCGAATTACTGCTTCCCAA
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
MERTK (10461)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000491689
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 10461 MERTK MER proto-oncogene, tyrosin... NM_006343.3 100% 100%
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
3069
ORF length:
2997
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catggggccg gccccgctgc cgctgctgct gggcctcttc ctccccgcgc
121tctggcgtag agctatcact gaggcaaggg aagaagccaa gccttacccg ctattcccgg
181gaccttttcc agggagcctg caaactgacc acacaccgct gttatccctt cctcacgcca
241gtgggtacca gcctgccttg atgttttcac caacccagcc tggaagacca catacaggaa
301acgtagccat tccccaggtg acctctgtcg aatcaaagcc cctaccgcct cttgccttca
361aacacacagt tggacacata atactttctg aacataaagg tgtcaaattt aattgctcaa
421tcagtgtacc taatatatac caggacacca caatttcttg gtggaaagat gggaaggaat
481tgcttggggc acatcatgca attacacagt tttatccaga tgatgaagtt acagcaataa
541tcgcttcctt cagcataacc agtgtgcagc gttcagacaa tgggtcgtat atctgtaaga
601tgaaaataaa caatgaagag atcgtgtctg atcccatcta catcgaagta caaggacttc
661ctcactttac taagcagcct gagagcatga atgtcaccag aaacacagcc ttcaacctca
721cctgtcaggc tgtgggcccg cctgagcccg tcaacatttt ctgggttcaa aacagtagcc
781gtgttaacga acagcctgaa aaatccccct ccgtgctaac tgttccaggc ctgacggaga
841tggcggtctt cagttgtgag gcccacaatg acaaagggct gaccgtgtcc aagggagtgc
901agatcaacat caaagcaatt ccctccccac caactgaagt cagcatccgt aacagcactg
961cacacagcat tctgatctcc tgggttcctg gttttgatgg atactccccg ttcaggaatt
1021gcagcattca ggtcaaggaa gctgatccgc tgagtaatgg ctcagtcatg atttttaaca
1081cctctgcctt accacatctg taccaaatca agcagctgca agccctggct aattacagca
1141ttggtgtttc ctgcatgaat gaaataggct ggtctgcagt gagcccttgg attctagcca
1201gcacgactga aggagcccca tcagtagcac ctttaaatgt cactgtgttt ctgaatgaat
1261ctagtgataa tgtggacatc agatggatga agcctccgac taagcagcag gatggagaac
1321tggtgggcta ccggatatcc cacgtgtggc agagtgcagg gatttccaaa gagctcttgg
1381aggaagttgg ccagaatggc agccgagctc ggatctctgt tcaagtccac aatgctacgt
1441gcacagtgag gattgcagcc gtcaccagag ggggagttgg gcccttcagt gatccagtga
1501aaatatttat ccctgcacac ggttgggtag attatgcccc ctcttcaact ccggcgcctg
1561gcaacgcaga tcctgtgctc atcatctttg gctgcttttg tggatttatt ttgattgggt
1621tgattttata catctccttg gccatcagaa aaagagtcca ggagacaaag tttgggaatg
1681cattcacaga ggaggattct gaattagtgg tgaattatat agcaaagaaa tccttctgtc
1741ggcgagccat tgaacttacc ttacatagct tgggagtcag tgaggaacta caaaataaac
1801tagaagatgt tgtgattgac aggaatcttc taattcttgg aaaaattctg ggtgaaggag
1861agtttgggtc tgtaatggaa ggaaatctta agcaggaaga tgggacctct ctgaaagtgg
1921cagtgaagac catgaagttg gacaactctt cacagcggga gatcgaggag tttctcagtg
1981aggcagcgtg catgaaagac ttcagccacc caaatgtcat tcgacttcta ggtgtgtgta
2041tagaaatgag ctctcaaggc atcccaaagc ccatggtaat tttacccttc atgaaatacg
2101gggacctgca tacttactta ctttattccc gattggagac aggaccaaag catattcctc
2161tgcagacact attgaagttc atggtggata ttgccctggg aatggagtat ctgagcaaca
2221ggaattttct tcatcgagat ttagctgctc gaaactgcat gttgcgagat gacatgactg
2281tctgtgttgc ggacttcggc ctctctaaga agatttacag tggcgattat taccgccaag
2341gccgcattgc taagatgcct gttaaatgga tcgccataga aagtcttgca gaccgagtct
2401acacaagtaa aagtgatgtg tgggcatttg gcgtgaccat gtgggaaata gctacgcggg
2461gaatgactcc ctatcctggg gtccagaacc atgagatgta tgactatctt ctccatggcc
2521acaggttgaa gcagcccgaa gactgcctgg atgaactgta tgaaataatg tactcttgct
2581ggagaaccga tcccttagac cgccccacct tttcagtatt gaggctgcag ctagaaaaac
2641tcttagaaag tttgcctgac gttcggaacc aagcagacgt tatttacgtc aatacacagt
2701tgctggagag ctctgagggc ctggcccagg gctccaccct tgctccactg gacttgaaca
2761tcgaccctga ctctataatt gcctcctgCA CTCCCCGCGC TGCCATCAGT GTGGTCACAG
2821CAGAAGTTCA TGACAGCAAA CCTCATGAAG GACGGTACAT CCTGAATGGG GGCAGTGAGG
2881AATGGGAAGA TCTGACTTCT GCCCCCTCTG CTGCAGTCAC AGCTGAAAAG AACAGTGTTT
2941TACCGGGGGA GAGACTTGTT AGGAATGGGG TCTCCTGGTC CCATTCGAGC ATGCTGCCCT
3001TGGGAAGCTC ATTGCCCGAT GAACTTTTGT TTGCTGACGA CTCCTCAGAA GGCTCAGAAG
3061TCCTGATGTG AGACCCAGCT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA
3121ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT
3181CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAAGG CTTCGAATTA CTGCTTCCCA
3241AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t

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