Construct: ORF TRCN0000491699
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004846.1_s317c1
- Derived from:
- ccsbBroadEn_07301
- DNA Barcode:
- TAGTGATTCCAATTCAGTTAATCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FBP2 (8789)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491699
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8789 | FBP2 | fructose-bisphosphatase 2 | NM_003837.4 | 99.7% | 99.7% | 256G>C;331C>A;723T>C |
| 2 | mouse | 14120 | Fbp2 | fructose bisphosphatase 2 | NM_007994.3 | 87.2% | 94.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1086
- ORF length:
- 1017
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gacggacaga agccccttcg aaaccgacat gctcaccctg acccgctacg 121 ttatggaaaa ggggcgtcag gccaaaggga ctggggagct cacccagctg ctgaactcaa 181 tgctgacggc catcaaagcc atctcctcgg ctgtgcgcaa ggccggtctg gcccacctgt 241 atggaatcgc aggaagcgtt aacgtgacgg gagatgaggt gaagaaactg gatgtgctat 301 ccaattccct ggtgatcaac atgctccaat cctcctatag tacctgcgtc ctggtctcag 361 aagagaataa ggacgccatc atcaccgcca aggagaagag ggggaaatac gtggtctgct 421 ttgacccact ggatggatct tccaatattg actgcctggc ctccatcgga accatctttg 481 ccatctatag aaagacctca gaggatgagc cttctgaaaa ggatgccctg cagtgtggcc 541 gcaatattgt ggccgcaggt tatgcgctgt acggtagtgc aaccctggtg gctctctcca 601 cagggcaagg cgtggacctc ttcatgcttg acccggctct tggtgaattt gtcctggtgg 661 aaaaagatgt caagattaag aagaaaggaa agatttacag cctgaatgag ggctatgcca 721 agtattttga tgcggccacc actgaatatg tgcagaaaaa gaaattccct gaggatggca 781 gtgctcccTA CGGGGCCAGG TATGTGGGCT CCATGGTGGC TGACGTGCAC CGCACCCTGG 841 TCTATGGAGG AATCTTCCTG TACCCAGCCA ACCAGAAGAG CCCTAAGGGC AAGCTCCGGC 901 TCCTGTATGA ATGCAATCCC GTGGCCTACA TCATTGAGCA GGCAGGAGGC TTGGCGACCA 961 CGGGGACCCA GCCTGTACTG GACGTGAAGC CCGAGGCAAT TCACCAGCGA GTCCCCCTCA 1021 TTCTGGGGTC ACCAGAGGAT GTGCAGGAAT ATCTCACCTG TGTGCAGAAA AATCAGGCAG 1081 GCAGCTTGCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1141 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1201 CTTGGCTTTA TATATCTTGT GGAAAGGACG ATAGTGATTC CAATTCAGTT AATCTACGCG 1261 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt