Construct: ORF TRCN0000491706
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021774.2_s317c1
- DNA Barcode:
- TATAGGGTCCGGACAACGGTTAAC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PIGZ (80235)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491706
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80235 | PIGZ | phosphatidylinositol glycan... | NM_025163.4 | 99.6% | 99.4% | (many diffs) |
| 2 | human | 80235 | PIGZ | phosphatidylinositol glycan... | XM_011513190.2 | 93.3% | 93.2% | (many diffs) |
| 3 | human | 80235 | PIGZ | phosphatidylinositol glycan... | XM_011513192.2 | 93.3% | 93.2% | (many diffs) |
| 4 | human | 80235 | PIGZ | phosphatidylinositol glycan... | XM_017007240.2 | 89.5% | 89.4% | (many diffs) |
| 5 | human | 80235 | PIGZ | phosphatidylinositol glycan... | XM_017007241.1 | 89.5% | 89.4% | (many diffs) |
| 6 | human | 80235 | PIGZ | phosphatidylinositol glycan... | XM_024453766.1 | 89.5% | 89.4% | (many diffs) |
| 7 | human | 80235 | PIGZ | phosphatidylinositol glycan... | XM_024453767.1 | 89.5% | 89.4% | (many diffs) |
| 8 | human | 80235 | PIGZ | phosphatidylinositol glycan... | XM_017007242.1 | 73.8% | 73.5% | (many diffs) |
| 9 | human | 80235 | PIGZ | phosphatidylinositol glycan... | XM_017007243.2 | 73.8% | 73.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1806
- ORF length:
- 1737
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gcagatctgt ggatccagcg tagcatctgt agcagctggg acatcattcc 121 aggttttggg cccggtgtgt tggcaacaac tggatctgaa gatggcagtc agggtgcttt 181 ggggtggtct cagcctgctc cgagtgctgt ggtgtctcct tccgcagacg ggctatgtgc 241 acccagatga gttcttccag tcccctgagg tgatggcaga ggacatcctg ggcgttcagg 301 ccgcgcggcc ctgggagttt taccccagca gctcctgccg ctcggtgctc ttccccctgc 361 tgatctccgg ttccaccttc tggctgctca ggctctggga ggagctgggg ccgtggcctg 421 gcctggtgag cggctatgcg ctgctggtgg ggcctcgact cctcctcact gccctttcct 481 ttgctctgga cggggccgtg taccacctgg ccccgccgat gggggcggat cgctggaacg 541 ccctggccct gctgtctggt tcctacgtca ccctggtctt ctacacaagg accttctcca 601 acaccattga gggactcctc ttcacgtggc tgctggtgct ggtatcctcc catgtaacgt 661 ggggccctac acgcaaggag ccggcgccgg gtccacggtg gcgcagctgg cttcttggag 721 gcattgtggc tgctggcttc ttcaaccggc ccacctttct ggcctttgct gtggtccccc 781 tctacctctg gggcactcgt ggagccacaa accctggttt gaagtctctg acccgggagg 841 ccctggtgct gctccctggg gcgaccctca cagcagcggt gtttgtggcc acggacagct 901 ggtatttctc cagccccgct acatccagga accttgtcct gacacctgtc aacttcctgc 961 actacaacct gaatccccaa aacctggcga gacatggcac gcacgcgcgg ctcactcacc 1021 tggcagtcaa cggcttcctg ctcttcgggg tgctgcatgc ccaggccctg caggctgcgt 1081 ggcaacagct gcaagtcggc ctccaggcct ctgcacaaat gggcctcctg agggcactgg 1141 gtgcccggag cctgctgtcc agccccaggt cctatctcct tctcctctac ttcatgcctc 1201 tggccctgct atctgccttt agccaccagg aggctcggtt cctgattccc ctcctggtcc 1261 ccctggtcct gctttgtagt ccacagacgc agcctgtgcc ttggaagggc actgtggtcc 1321 tcttcaacgc cctcggtgcc ctcctcttcg gctgcctgca tcaggggggc ctggtgcctg 1381 gcctggagta cctggagcag gtggtccatg cccctgtgct cccaagcaca cccacccact 1441 acacactcct cttcactcac acctacatgc ccccccggca cctcctacac ctCCCAGGCC 1501 TGGGGGCACC AGTGGAGGTG GTGGACATAG GGGGGACTGA GGACTGGGCC CTGTGCCAAA 1561 CCCTGAAAAG CTTCACCAGA CAACCAGCCT GCCAAGTGGC TGGTGGGCCA TGGCTCTGCC 1621 GCCTCTTTGT GGTAACCCCT GGCACCACCA GGCGTGCCGT GGAGAAGTGC AGCTTCCCCT 1681 TCAAGAATGA AACACTTTTA TTTCCCCATC TGACCCTGGA GGATCCACCA GCCCTGTCCT 1741 CCTTGCTGAG TGGGGCTTGG AGGGACCACC TCAGTCTTCA CATTGTGGAG CTGGGGGAAG 1801 AAACCTAGAA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1861 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1921 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATATAGG GTCCGGACAA CGGTTAACAC 1981 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt