Construct: ORF TRCN0000491710
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006287.1_s317c1
- Derived from:
- ccsbBroadEn_05941
- DNA Barcode:
- CAAGGAAAAAAATGGTCTAGTTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CBR3 (874)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491710
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 874 | CBR3 | carbonyl reductase 3 | NM_001236.4 | 99.7% | 100% | 255C>T;606G>A |
| 2 | human | 874 | CBR3 | carbonyl reductase 3 | XM_011529772.2 | 49.5% | 47.8% | (many diffs) |
| 3 | mouse | 109857 | Cbr3 | carbonyl reductase 3 | NM_173047.3 | 83.9% | 85.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 897
- ORF length:
- 831
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc gtcctgcagc cgcgtggcgc tggtgaccgg ggccaacagg ggcatcggct 121 tggccatcgc gcgcgaactg tgccgacagt tctctgggga tgtggtgctc accgcgcggg 181 acgtggcgcg gggccaggcg gccgtgcagc agctgcaggc ggagggcctg agcccgcgct 241 tccaccaact ggacatcgac gacttgcaga gcatccgcgc cctgcgcgac ttcctgcgca 301 aggagtacgg ggggctcaat gtactggtca acaacgcggc cgtcgccttc aagagtgatg 361 atccaatgcc ctttgacatt aaagctgaga tgacactgaa gacaaatttt tttgccacta 421 gaaacatgtg caacgagtta ctgccgataa tgaaacctca tgggagagtg gtgaatatca 481 gtagtttgca gtgtttaagg gcttttgaaa actgcagtga agatctgcag gaaaggttcc 541 acagtgagac actcacagaa ggagacctgg tggatcTCAT GAAAAAGTTT GTGGAGGACA 601 CAAAAAATGA GGTGCATGAG AGGGAAGGCT GGCCCAACTC ACCTTATGGG GTGTCCAAGT 661 TGGGGGTCAC AGTCTTATCG AGGATCCTGG CCAGGCGTCT GGATGAGAAG AGGAAAGCTG 721 ACAGGATTCT GGTGAATGCG TGCTGCCCAG GACCAGTGAA GACAGACATG GATGGGAAAG 781 ACAGCATCAG GACTGTGGAG GAGGGGGCTG AGACCCCTGT CTACTTGGCC CTCTTGCCTC 841 CAGATGCCAC TGAGCCACAA GGCCAGTTGG TCCATGACAA AGTTGTGCAA AACTGGTACC 901 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 961 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1021 ATATATCTTG TGGAAAGGAC GACAAGGAAA AAAATGGTCT AGTTTCACGC GTTAAGTCga 1081 caatcaacct ctggattaca aaatttgtga aagatt