Construct: ORF TRCN0000491716
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005916.1_s317c1
- Derived from:
- ccsbBroadEn_03083
- DNA Barcode:
- ACTCGGCCCTCCATACGACGGATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MYLIP (29116)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491716
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29116 | MYLIP | myosin regulatory light cha... | NM_013262.4 | 100% | 100% | |
| 2 | human | 29116 | MYLIP | myosin regulatory light cha... | XM_017010789.1 | 94.5% | 92.8% | (many diffs) |
| 3 | human | 29116 | MYLIP | myosin regulatory light cha... | XM_005249032.2 | 87.6% | 87.6% | 660_661ins165 |
| 4 | human | 29116 | MYLIP | myosin regulatory light cha... | XM_005249033.3 | 59.3% | 59.3% | 0_1ins543 |
| 5 | mouse | 218203 | Mylip | myosin regulatory light cha... | NM_153789.3 | 88.9% | 96.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1401
- ORF length:
- 1335
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct gtgttatgtg acgaggccgg acgcggtgct gatggaggtg gaggtggagg 121 cgaaagccaa cggcgaggac tgcctcaacc aggtgtgcag gcgactggga atcatagaag 181 ttgactattt tggactgcag tttacgggta gcaaaggtga aagtttatgg ctaaacctga 241 gaaaccggat ctcccagcag atggatgggc tagcccctta caggcttaaa cttagagtca 301 agttcttcgt ggagcctcat ctcatcttac aggagcagac taggcatatc tttttcttgc 361 acatcaagga ggccctcttg gcaggccacc tcttgtgttc cccagagcag gcagtggaac 421 tcagtgccct cctggcccag accaagtttg gagactacaa ccagaacact gccaagtata 481 actatgagga gctctgtgcc aaggagctct cctctgccac cttgaacagc attgttgcaa 541 aacataagga gttggagggg accagccagg cttcagctga ataccaagtt ttgcagattg 601 tgtcggcaat ggaaaactat ggcatagaat ggcattctgt gcgggatagc gaagggcaga 661 aactgctcat tggggttgga cctgaaggaa tctcaatttg taaagatgac tttagcccaa 721 ttaataggat agcttatcct gtggtgcaga tggccaccca gtcaggaaag aatgtatatt 781 tgacggtcac caaggaatct gggaacagca tcgtgctctt gtttaaaatg atcagcacca 841 gggcggccag cgggctctac cgagcgataa cagagacgca cgcattctac aggtgtgaca 901 cagtgaccag cgccgtgatg atgcagtata gccgtgactt gaagggccac ttggcatctc 961 tgtttctgaa tgaaaacatt aaccttggca agaaatatgt ctttgatatt aaaagaacat 1021 caaaggaggt gtatgaccat gccaggaggg ctctgtacaa tgctGGCGTT GTGGACCTCG 1081 TTTCAAGAAA CAACCAGAGC CCTTCACACT CGCCTCTGAA GTCCTCAGAA AGCAGCATGA 1141 ACTGCAGCAG CTGCGAGGGC CTCAGCTGCC AGCAGACCCG GGTGCTGCAG GAGAAGCTAC 1201 GCAAGCTGAA GGAAGCCATG CTGTGCATGG TGTGCTGCGA GGAGGAGATC AACTCCACCT 1261 TCTGTCCCTG TGGCCACACT GTGTGCTGTG AGAGCTGCGC CGCCCAGCTA CAGTCATGTC 1321 CCGTCTGCAG GTCGCGTGTG GAGCATGTCC AGCACGTCTA TCTGCCAACG CACACCAGTC 1381 TTCTCAATCT GACTGTAATC TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1441 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1501 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAACTC GGCCCTCCAT 1561 ACGACGGATG ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt