Construct: ORF TRCN0000491720
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019844.2_s317c1
- DNA Barcode:
- TAGACATTTATATTTTAGCAAATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DRD2 (1813)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491720
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1813 | DRD2 | dopamine receptor D2 | NM_000795.4 | 99.9% | 99.7% | 1329_1330insG |
| 2 | human | 1813 | DRD2 | dopamine receptor D2 | XM_017017296.2 | 99.9% | 99.7% | 1329_1330insG |
| 3 | human | 1813 | DRD2 | dopamine receptor D2 | NM_016574.3 | 93.3% | 93.2% | 722_723ins87;1242_1243insG |
| 4 | mouse | 13489 | Drd2 | dopamine receptor D2 | NM_010077.2 | 90.5% | 95.2% | (many diffs) |
| 5 | mouse | 13489 | Drd2 | dopamine receptor D2 | XM_006509996.3 | 84.2% | 88.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1401
- ORF length:
- 1332
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggatccactg aatctgtcct ggtatgatga tgatctggag aggcagaact 121 ggagccggcc cttcaacggg tcagacggga aggcggacag accccactac aactactatg 181 ccacactgct caccctgctc atcgctgtca tcgtcttcgg caacgtgctg gtgtgcatgg 241 ctgtgtcccg cgagaaggcg ctgcagacca ccaccaacta cctgatcgtc agcctcgcag 301 tggccgacct cctcgtcgcc acactggtca tgccctgggt tgtctacctg gaggtggtag 361 gtgagtggaa attcagcagg attcactgtg acatcttcgt cactctggac gtcatgatgt 421 gcacggcgag catcctgaac ttgtgtgcca tcagcatcga caggtacaca gctgtggcca 481 tgcccatgct gtacaatacg cgctacagct ccaagcgccg ggtcaccgtc atgatctcca 541 tcgtctgggt cctgtccttc accatctcct gcccactcct cttcggactc aataacgcag 601 accagaacga gtgcatcatt gccaacccgg ccttcgtggt ctactcctcc atcgtctcct 661 tctacgtgcc cttcattgtc accctgctgg tctacatcaa gatctacatt gtcctccgca 721 gacgccgcaa gcgagtcaac accaaacgca gcagccgagc tttcagggcc cacctgaggg 781 ctccactaaa gggcaactgt actcaccccg aggacatgaa actctgcacc gttatcatga 841 agtctaatgg gagtttccca gtgaacaggc ggagagtgga ggctgcccgg cgagcccagg 901 agctggagat ggagatgctc tccagcacca gcccacccga gaggacccgg tacagcccca 961 tcccacccag ccaccaccag ctgactctcc ccGACCCGTC CCACCATGGT CTCCACAGCA 1021 CTCCCGACAG CCCCGCCAAA CCAGAGAAGA ATGGGCATGC CAAAGACCAC CCCAAGATTG 1081 CCAAGATCTT TGAGATCCAG ACCATGCCCA ATGGCAAAAC CCGGACCTCC CTCAAGACCA 1141 TGAGCCGTAG GAAGCTCTCC CAGCAGAAGG AGAAGAAAGC CACTCAGATG CTCGCCATTG 1201 TTCTCGGCGT GTTCATCATC TGCTGGCTGC CCTTCTTCAT CACACACATC CTGAACATAC 1261 ACTGTGACTG CAACATCCCG CCTGTCCTGT ACAGCGCCTT CACGTGGCTG GGCTATGTCA 1321 ACAGCGCCGT GAACCCCATC ATCTACACCA CCTTCAACAT TGAGTTCCGC AAGGCCTTCC 1381 TGAAGATCCT CCACTGCGAC CCAGCTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1441 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1501 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATAGACAT TTATATTTTA 1561 GCAAATAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt