Construct: ORF TRCN0000491735
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012270.1_s317c1
- Derived from:
- ccsbBroadEn_00618
- DNA Barcode:
- ACTATATATTTCGAAGCCTGCCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NIPSNAP2 (2631)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491735
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2631 | NIPSNAP2 | nipsnap homolog 2 | NM_001483.3 | 100% | 100% | |
| 2 | human | 2631 | NIPSNAP2 | nipsnap homolog 2 | NM_001202469.2 | 86.3% | 79% | 232_233ins46;326_327ins71 |
| 3 | mouse | 14467 | Nipsnap2 | nipsnap homolog 2 | NM_008095.4 | 84.5% | 90.5% | (many diffs) |
| 4 | mouse | 14467 | Nipsnap2 | nipsnap homolog 2 | XR_377052.3 | 32.9% | (many diffs) | |
| 5 | mouse | 14467 | Nipsnap2 | nipsnap homolog 2 | XR_377051.3 | 32.7% | (many diffs) | |
| 6 | mouse | 14467 | Nipsnap2 | nipsnap homolog 2 | XR_377054.3 | 31.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 924
- ORF length:
- 858
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcgcgagtg ctgcgcgccc gcggagcggc ctgggccggc ggcctcctgc 121 agcgggcggc cccctgcagc ctcctgccca ggctccggac atggacatct tccagcaaca 181 gatctcgaga agacagctgg ctaaaatcct tatttgtccg gaaagttgat ccaagaaaag 241 atgcccactc caatctccta gccaaaaagg aaacaagcaa tctatacaaa ttacagtttc 301 acaatgttaa accggaatgc ctagaagcat acaacaaaat ttgtcaagag gtgttgccaa 361 agattcacga agataaacac tacccttgta ctttggtggg gacttggaac acgtggtatg 421 gcgagcagga ccaagctgtc cacctctgga ggtatgaagg aggctatcca gccctcacag 481 aagtcatgaa taaactcaga gaaaataagg aatttttGGA ATTTCGTAAG GCAAGAAGTG 541 ACATGCTTCT CTCCAGGAAG AATCAGCTCC TGTTGGAGTT CAGTTTCTGG AATGAGCCTG 601 TGCCAAGATC CGGACCTAAT ATATATGAAC TCAGGTCTTA CCAACTCCGA CCAGGAACCA 661 TGATTGAATG GGGCAATTAC TGGGCTCGTG CAATCCGCTT CAGACAGGAT GGTAACGAAG 721 CCGTCGGAGG ATTCTTCTCT CAGATTGGGC AGCTGTACAT GGTGCACCAT CTTTGGGCTT 781 ACAGGGATCT TCAGACCAGG GAAGACATAC GGAATGCAGC ATGGCACAAA CATGGCTGGG 841 AGGAATTGGT ATATTACACA GTTCCACTTA TTCAGGAAAT GGAATCCAGA ATCATGATCC 901 CACTGAAGAC CTCGCCCCTC CAGTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 961 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1021 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA CTATATATTT 1081 CGAAGCCTGC CCTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1141 att