Construct: ORF TRCN0000491740

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020518.2_s317c1
DNA Barcode:
GACTTCGACTGTCGATACGGATGT
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
PAPSS1 (9061)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000491740
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9061 PAPSS1 3'-phosphoadenosine 5'-phos... XM_011532400.2 100% 100%
2 human 9061 PAPSS1 3'-phosphoadenosine 5'-phos... XM_011532401.1 100% 100%
3 human 9061 PAPSS1 3'-phosphoadenosine 5'-phos... NM_005443.5 96.6% 96.6% 1_63del
4 mouse 23971 Papss1 3'-phosphoadenosine 5'-phos... NM_001289477.1 87.8% 98.6% (many diffs)
5 mouse 23971 Papss1 3'-phosphoadenosine 5'-phos... NM_001289478.1 87.8% 98.6% (many diffs)
6 mouse 23971 Papss1 3'-phosphoadenosine 5'-phos... NM_011863.2 84.8% 95.3% (many diffs)
7 mouse 23971 Papss1 3'-phosphoadenosine 5'-phos... NM_001289479.1 80.5% 90.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
1881
ORF length:
1809
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catgcagaga gcaaccaatg tcacctacca agcccatcat gtcagcagga
121acaagagagg tcaggtggtg gggaccagag gtggctttcg tggttgcaca gtttggctaa
181caggcttgtc tggagcggga aagactactg tgagcatggc cttggaggag tacctggttt
241gtcatggtat tccatgctac actctggatg gtgacaatat tcgtcaaggt ctcaataaaa
301atcttggctt tagtcctgaa gacagagaag agaatgttcg acgcatcgca gaagttgcta
361aactgtttgc agatgctggc ttagtgtgca tcacaagttt catatcacct tacactcagg
421atcgcaacaa tgcaaggcaa attcatgaag gtgcaagttt accgtttttt gaagtatttg
481ttgatgctcc tctgcatgtt tgtgaacaga gggatgtcaa aggactctac aaaaaagccc
541gggcaggaga aattaaaggt ttcactggga tcgattctga atatgaaaag ccagaggccc
601ctgagttggt gctgaaaaca gactcctgtg atgtaaatga ctgtgtccag caagttgtgg
661aacttctaca ggaacgggat attgtacctg tggatgcatc ttatgaagta aaagaactat
721atgtgccaga aaataaactt catttggcaa aaacagatgc ggaaacatta ccagcactga
781aaattaataa agtggatatg cagtgggtgc aggttttggc agaaggttgg gcaaccccat
841tgaatggctt tatgagagag agggagtact tgcagtgcct tcattttgat tgtcttctgg
901atggaggtgt cattaacttg tcagtaccta tagttctgac tgcgactcat gaagataaag
961agaggctgga cggctgtaca gcatttgctc tgatgtatga gggccgccgt gtggccattc
1021ttcgcaatcc agagtttttt gagcacagga aagaggagcg ctgtgccaga cagtggggaa
1081cgacatgcaa gaaccacccc tatattaaga tggtgatgga acaaggagat tggctgattg
1141gaggagatct tcaagtcttg gatcgagttt attggaatga tggtcttgat cagtatcgtc
1201ttactcctac tgagctaaag cagaaattta aagatatgaa tgctgatgct gtctttgcat
1261ttcaactacg caacccagtg cacaatggac atgccctgtt aatgcaggat acccataagc
1321aacttctaga gaggggctac cggcgccctg tcctcctcct ccaccctctg ggtggctgga
1381caaaggatga cgatgttcct ttgatgtggc gtatgaagca gcatgctgca gtgttggagg
1441aaggagttct gaatccTGAG ACGACAGTGG TGGCCATCTT CCCATCTCCC ATGATGTATG
1501CTGGACCAAC TGAGGTCCAG TGGCATTGCA GAGCACGGAT GGTTGCAGGA GCCAACTTTT
1561ACATTGTTGG ACGAGACCCT GCTGGCATGC CTCATCCAGA AACAGGGAAG GATCTTTATG
1621AGCCAAGTCA TGGTGCCAAA GTGCTGACGA TGGCCCCTGG TTTAATCACT TTGGAAATAG
1681TTCCCTTTCG AGTTGCAGCT TACAACAAGA AAAAGAAGCG TATGGACTAC TATGACTCTG
1741AACACCATGA AGACTTTGAA TTTATTTCAG GAACACGAAT GCGCAAACTT GCTCGAGAAG
1801GCCAGAAACC ACCTGAAGGT TTCATGGCTC CCAAGGCTTG GACCGTGCTG ACAGAATACT
1861ACAAATCCTT GGAGAAAGCT TAGAACCCAG CTTTCTTGTA CAAAGTGGTT GATATCGGTA
1921AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC
1981TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAG ACTTCGACTG
2041TCGATACGGA TGTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag
2101att

Download FASTA (ORF) (Full)

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