Construct: ORF TRCN0000491740
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020518.2_s317c1
- DNA Barcode:
- GACTTCGACTGTCGATACGGATGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PAPSS1 (9061)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491740
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9061 | PAPSS1 | 3'-phosphoadenosine 5'-phos... | XM_011532400.2 | 100% | 100% | |
| 2 | human | 9061 | PAPSS1 | 3'-phosphoadenosine 5'-phos... | XM_011532401.1 | 100% | 100% | |
| 3 | human | 9061 | PAPSS1 | 3'-phosphoadenosine 5'-phos... | NM_005443.5 | 96.6% | 96.6% | 1_63del |
| 4 | mouse | 23971 | Papss1 | 3'-phosphoadenosine 5'-phos... | NM_001289477.1 | 87.8% | 98.6% | (many diffs) |
| 5 | mouse | 23971 | Papss1 | 3'-phosphoadenosine 5'-phos... | NM_001289478.1 | 87.8% | 98.6% | (many diffs) |
| 6 | mouse | 23971 | Papss1 | 3'-phosphoadenosine 5'-phos... | NM_011863.2 | 84.8% | 95.3% | (many diffs) |
| 7 | mouse | 23971 | Papss1 | 3'-phosphoadenosine 5'-phos... | NM_001289479.1 | 80.5% | 90.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1881
- ORF length:
- 1809
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgcagaga gcaaccaatg tcacctacca agcccatcat gtcagcagga 121 acaagagagg tcaggtggtg gggaccagag gtggctttcg tggttgcaca gtttggctaa 181 caggcttgtc tggagcggga aagactactg tgagcatggc cttggaggag tacctggttt 241 gtcatggtat tccatgctac actctggatg gtgacaatat tcgtcaaggt ctcaataaaa 301 atcttggctt tagtcctgaa gacagagaag agaatgttcg acgcatcgca gaagttgcta 361 aactgtttgc agatgctggc ttagtgtgca tcacaagttt catatcacct tacactcagg 421 atcgcaacaa tgcaaggcaa attcatgaag gtgcaagttt accgtttttt gaagtatttg 481 ttgatgctcc tctgcatgtt tgtgaacaga gggatgtcaa aggactctac aaaaaagccc 541 gggcaggaga aattaaaggt ttcactggga tcgattctga atatgaaaag ccagaggccc 601 ctgagttggt gctgaaaaca gactcctgtg atgtaaatga ctgtgtccag caagttgtgg 661 aacttctaca ggaacgggat attgtacctg tggatgcatc ttatgaagta aaagaactat 721 atgtgccaga aaataaactt catttggcaa aaacagatgc ggaaacatta ccagcactga 781 aaattaataa agtggatatg cagtgggtgc aggttttggc agaaggttgg gcaaccccat 841 tgaatggctt tatgagagag agggagtact tgcagtgcct tcattttgat tgtcttctgg 901 atggaggtgt cattaacttg tcagtaccta tagttctgac tgcgactcat gaagataaag 961 agaggctgga cggctgtaca gcatttgctc tgatgtatga gggccgccgt gtggccattc 1021 ttcgcaatcc agagtttttt gagcacagga aagaggagcg ctgtgccaga cagtggggaa 1081 cgacatgcaa gaaccacccc tatattaaga tggtgatgga acaaggagat tggctgattg 1141 gaggagatct tcaagtcttg gatcgagttt attggaatga tggtcttgat cagtatcgtc 1201 ttactcctac tgagctaaag cagaaattta aagatatgaa tgctgatgct gtctttgcat 1261 ttcaactacg caacccagtg cacaatggac atgccctgtt aatgcaggat acccataagc 1321 aacttctaga gaggggctac cggcgccctg tcctcctcct ccaccctctg ggtggctgga 1381 caaaggatga cgatgttcct ttgatgtggc gtatgaagca gcatgctgca gtgttggagg 1441 aaggagttct gaatccTGAG ACGACAGTGG TGGCCATCTT CCCATCTCCC ATGATGTATG 1501 CTGGACCAAC TGAGGTCCAG TGGCATTGCA GAGCACGGAT GGTTGCAGGA GCCAACTTTT 1561 ACATTGTTGG ACGAGACCCT GCTGGCATGC CTCATCCAGA AACAGGGAAG GATCTTTATG 1621 AGCCAAGTCA TGGTGCCAAA GTGCTGACGA TGGCCCCTGG TTTAATCACT TTGGAAATAG 1681 TTCCCTTTCG AGTTGCAGCT TACAACAAGA AAAAGAAGCG TATGGACTAC TATGACTCTG 1741 AACACCATGA AGACTTTGAA TTTATTTCAG GAACACGAAT GCGCAAACTT GCTCGAGAAG 1801 GCCAGAAACC ACCTGAAGGT TTCATGGCTC CCAAGGCTTG GACCGTGCTG ACAGAATACT 1861 ACAAATCCTT GGAGAAAGCT TAGAACCCAG CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1921 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1981 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAG ACTTCGACTG 2041 TCGATACGGA TGTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 2101 att