Construct: ORF TRCN0000491744
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020088.2_s317c1
- DNA Barcode:
- GGGGCTGCGAGCAGTATAGCGGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ERN1 (2081)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491744
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2081 | ERN1 | endoplasmic reticulum to nu... | NM_001433.5 | 99.5% | 99.7% | (many diffs) |
| 2 | human | 2081 | ERN1 | endoplasmic reticulum to nu... | XM_017024347.2 | 95.3% | 94.7% | (many diffs) |
| 3 | human | 2081 | ERN1 | endoplasmic reticulum to nu... | XM_017024348.2 | 92% | 91.4% | (many diffs) |
| 4 | mouse | 78943 | Ern1 | endoplasmic reticulum (ER) ... | NM_023913.2 | 88.3% | 92.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 96
- ORF end:
- 3033
- ORF length:
- 2937
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggctccgc ggccgccccc ttcaccaagc ttgccatgcc tgcacgacga ctgctgctgc 121 tgctgacgct gctgctgccc ggcctcggga tttttggaag taccagcaca gtgacgcttc 181 ctgaaacctt gttgtttgtg tcaacgctgg atggaagttt gcatgctgtc agcaagagga 241 caggctcaat caaatggact ttaaaagaag atccagtcct gcaggtccca acacatgtgg 301 aagagcctgc ctttctccca gatcctaatg atggcagcct gtatacgctt ggaagcaaga 361 ataatgaagg cctgacgaaa cttcctttta ccatcccaga attggtgcag gcatccccat 421 gccgaagttc agatggaatc ctctacatgg gtaaaaagca ggacatctgg tatgttattg 481 acctcctgac cggagagaag cagcagactt tgtcatcggc ctttgcagat agtctctgcc 541 catcaacctc tcttctgtat cttgggcgaa cagaatacac catcaccatg tacgacacca 601 aaacccgaga gctccggtgg aatgccacct actttgacta tgcggcctca ctgcctgagg 661 acgacgtgga ctacaagatg tcccactttg tgtccaatgg tgatgggctg gtggtgactg 721 tggacagtga atctggggac gtcctgtgga tccaaaacta cgcctcccct gtggtggcct 781 tttatgtctg gcagcgggag ggtctgagga aggtgatgca catcaatgtc gctgtggaga 841 ccctgcgcta tctgaccttc atgtctgggg aggtggggcg catcacaaag tggaagtacc 901 cgttccccaa ggagacagag gccaagagca agctgacgcc cactctgtat gttgggaaat 961 actctaccag cctctatgcc tctccctcaa tggtacacga gggggttgct gtcgtgcccc 1021 gcggcagcac acttcctttg ctggaagggc cccagactga tggcgtcacc atcggggaca 1081 agggggagtg tgtgatcacg cccagcacgg acgtcaagtt tgatcccgga ctcaaaagca 1141 agaacaagct caactacttg aggaattact ggcttctgat aggacaccat gaaaccccac 1201 tgtctgcgtc taccaagatg ctggagagat ttcccaacaa tctacccaaa catcgggaaa 1261 atgtgattcc tgctgattca gagaaaaaga gctttgagga agttatcaac ctggttgacc 1321 agacttcaga aaacgcacct accaccgtgt ctcgggatgt ggaggagaag cccgcccatg 1381 cccctgcccg gcccgaggcc cccgtggact ccatgcttaa ggacatggct accatcatcc 1441 tgagcacctt cctgctgatt ggctgggtgg ccttcatcat cacctatccc ctgagcatgc 1501 atcagcagca gcagctccag caccagcagt tccagaagga actggagaag atccagctcc 1561 tgcagcagca gcagcagcag ctgcccttcc acccacctgg agacacggct caggacggcg 1621 agctcctgga cacgtctggc ccgtactcag agagctcggg caccagcagc cccagcacgt 1681 cccccagggc ctccaaccac tcgctctgct ccggcagctc tgcctccaag gctggcagca 1741 gcccctccct ggaacaagac gatggagatg aggaaaccag cgtggtgata gttgggaaaa 1801 tttccttctg tcccaaggat gtcctgggcc atggagctga gggcacaatt gtgtaccggg 1861 gcatgtttga caaccgcgac gtggccgtga agaggatcct ccccgagtgt tttagcttcg 1921 cagaccgtga ggtccagctg ttgcgagaat cggatgagca cccgaacgtg atccgctact 1981 tctgcacgga gaaggaccgg caattccagt acattgccat cgagctgtgt gcagccaccc 2041 tgcaagagta tgtggagcag aaggactttg cgcatctcgg cctggagccc atcaccttgc 2101 tgcagcagac cacctcgggc ctggcccacc tccactccct caacatcgtt cacagagacc 2161 taaagccaca caacatcctc atatccatgc ccaatgcaca cggcaagatc aaggccatga 2221 tctccgactt tggcctctgc aagaagctgg cagtgggcag acacagtttc agccgccgat 2281 ctggggtgcc tggcacagaa ggctggatcg ctccagagat gctgagcgaa gactgtaagg 2341 agaaccctac ctacacggtg gacatctttt ctgcaggctg cgtcttttac tacgtaatct 2401 ctgagggcag ccaccctttt ggcaagtccc tgcagcggca ggccaacatc ctcctgggtg 2461 cctgcagcct tgactgcttg cacccagaga agcacgaaga cgtcattgca cgtgaattga 2521 tagagaagat gattgcgatg gatcctcaga aacgcccctc agcgaagcac gtgctcaaac 2581 acccgttctt ctggagccta gagaagcagc tccagttctt ccaggacgtg agcgacagaa 2641 tagaaaagga atccctggat ggcccgatcg tgaagcagtt agagagaggc gggagagccg 2701 tggtgaagat ggactggcgg gagaacatca ctgtccccct ccagacagac ctgcgtaaat 2761 tcaggaccta taaaggtGGT TCTGTCAGAG ATCTCCTCCG AGCCATGAGA AATAAGAAGC 2821 ACCACTACCG GGAGCTGCCT GCAGAGGTGC GGGAGACGCT GGGGTCCCTC CCCGACGACT 2881 TCGTGTGCTA CTTCACGTCT CGCTTCCCCC ACCTCCTCGC ACACACCTAC CGGGCCATGG 2941 AGCTGTGCAG CCACGAGAGA CTCTTCCAGC CCTACTACTT CCACGAGCCC CCAGAGCCCC 3001 AGCCCCCAGT GACTCCAGAC GCCCTCTCTA GAAAGGGTGG GCGCGCCGAC CCAGCTTTCT 3061 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 3121 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 3181 GTGGAAAGGA CGAGGGGCTG CGAGCAGTAT AGCGGCCACG CGTTAAGTCg acaatcaacc 3241 tctggattac aaaatttgtg aaagatt