Construct: ORF TRCN0000491752
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004051.1_s317c1
- Derived from:
- ccsbBroadEn_04389
- DNA Barcode:
- CCAACGTTTTATCAGGTAAGACAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HOOK3 (84376)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491752
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84376 | HOOK3 | hook microtubule tethering ... | NM_032410.4 | 100% | 100% | |
| 2 | mouse | 320191 | Hook3 | hook microtubule tethering ... | NM_207659.3 | 90.4% | 97.7% | (many diffs) |
| 3 | mouse | 320191 | Hook3 | hook microtubule tethering ... | XM_006509148.2 | 90.1% | 97.5% | (many diffs) |
| 4 | mouse | 320191 | Hook3 | hook microtubule tethering ... | XM_006509150.2 | 85.1% | 92% | (many diffs) |
| 5 | mouse | 320191 | Hook3 | hook microtubule tethering ... | XM_006509149.3 | 84.3% | 89.4% | (many diffs) |
| 6 | mouse | 320191 | Hook3 | hook microtubule tethering ... | XM_006509151.3 | 69.3% | 73.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2220
- ORF length:
- 2154
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt cagcgtagag tcgctggagc gggcggagct gtgcgagagc ctcctcactt 121 ggatccagac atttaatgtg gatgcaccat gccagaccgt ggaagattta acgaatgggg 181 ttgtgatggc ccaggttctt caaaagatag atcctgcata ttttgatgaa aattggctaa 241 acagaatcaa aactgaagta ggagataatt ggaggctaaa gataagcaat ttaaagaaaa 301 ttttaaaagg aatcttggat tataatcatg agattttagg acagcaaatt aatgacttta 361 cccttcctga tgtgaacctt attggggagc attctgatgc agcagagctt ggaaggatgc 421 ttcagctcat cttaggctgt gctgtgaact gtgaacagaa gcaagagtac atccaagcca 481 ttatgatgat ggaggaatct gttcaacatg ttgtcatgac agccattcaa gagctgatga 541 gtaaagaatc tcctgtctct gctggaaatg atgcctatgt tgaccttgat cgtcagctga 601 agaaaactac agaggaacta aatgaagctt tgtcagcaaa ggaagaaatt gctcaaagat 661 gccatgaact ggatatgcag gttgcagcat tgcaggaaga gaaaagtagt ttgttggcag 721 agaatcaggt attaatggaa agactcaatc aatctgattc tatagaagac cctaacagtc 781 cagcaggaag aaggcatttg cagctccaga ctcaattaga acagctccaa gaagaaacat 841 tcagactaga agcagccaaa gatgattatc gaatacgttg tgaagagtta gaaaaggaga 901 tctctgaact tcggcaacag aatgatgaac tgaccacttt ggcagatgaa gctcagtctc 961 tgaaagatga gatcgacgtg ctgagacatt cttctgataa agtatctaaa ctagaaggtc 1021 aagtagaatc ttataaaaag aagctagaag accttggtga tttaaggcgg caggttaaac 1081 tcttagaaga gaagaatacc atgtatatgc agaatactgt cagtctagag gaagagttaa 1141 gaaaggccaa cgcagcgcga agtcaacttg aaacctacaa gagacaggta gtagaactac 1201 aaaacagatt atccgaagaa tcaaagaaag cagataaact agattttgaa tataagcggc 1261 taaaagaaaa agttgacagt cttcaaaaag aaaaggacag gctgagaaca gaaagggatt 1321 ctctgaagga aaccattgaa gagcttcgtt gtgtacaagc tcaagaaggg cagctcacaa 1381 cacaagggtt aatgcctctt ggaagtcagg agtcttcaga cagtctagct gcagagattg 1441 ttacacctga aatcagggag aaacttattc gtcttcagca tgagaataag atgttaaagc 1501 ttaaccaaga aggttcggac aatgaaaaaa tagccttatt gcagagcctt ctagatgatg 1561 caaatctacg caagaatgaa ctggagacag agaataggct ggtgaatcaa agacttctgg 1621 aagtacagtc acaagttgaa gaattacaaa aatctttaca ggatcaaggc tcaaaagcag 1681 aagattcagt ccttctaaaa aagaagcttg aagaacatct agagaagctg catgaggcca 1741 ataatgaact acagaagaag agagccatta ttgaagatct cgagccaaga tttaacaaca 1801 gctccttaaa aattgaagaa ttacaagaag ctttacgaaa gaaagaggaa gaaatgaagc 1861 aaatggaaga acgataCAAA AAATACTTAG AGAAAGCCAA AAGTGTCATC CGTACTTTAG 1921 ATCCTAAACA GAATCAAGGA GCAGCACCAG AAATACAAGC TCTTAAAAAT CAGCTCCAGG 1981 AACGAGACCG ACTGTTCCAC TCATTAGAGA AAGAATATGA GAAAACAAAG AGTCAGAGAG 2041 AGATGGAAGA GAAATATATT GTTAGTGCCT GGTACAATAT GGGAATGACC CTGCATAAAA 2101 AGGCAGCTGA AGATAGACTG GCAAGCACAG GCTCAGGGCA GTCATTTCTG GCGAGGCAGA 2161 GGCAAGCGAC CAGCAGCAGA AGATCATACC CAGGCCACGT GCAGCCGGCC ACAGCAAGGT 2221 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 2281 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 2341 TTTATATATC TTGTGGAAAG GACGACCAAC GTTTTATCAG GTAAGACATA CGCGTTAAGT 2401 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt