Construct: ORF TRCN0000491755
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017094.2_s317c1
- Derived from:
- ccsbBroadEn_12149
- DNA Barcode:
- CCTTTACTTAGGCAGGTTAATAGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SPATA6L (55064)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491755
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55064 | SPATA6L | spermatogenesis associated ... | XM_017014885.1 | 50% | 49% | (many diffs) |
| 2 | human | 55064 | SPATA6L | spermatogenesis associated ... | NM_001353484.2 | 39.9% | 39.1% | (many diffs) |
| 3 | human | 55064 | SPATA6L | spermatogenesis associated ... | NM_001353485.2 | 39.9% | 39.1% | (many diffs) |
| 4 | human | 55064 | SPATA6L | spermatogenesis associated ... | XM_006716795.2 | 39.9% | 39.1% | (many diffs) |
| 5 | human | 55064 | SPATA6L | spermatogenesis associated ... | XM_017014882.2 | 39.9% | 39.1% | (many diffs) |
| 6 | human | 55064 | SPATA6L | spermatogenesis associated ... | XM_017014883.2 | 39.9% | 39.1% | (many diffs) |
| 7 | human | 55064 | SPATA6L | spermatogenesis associated ... | XM_024447596.1 | 39.9% | 39.1% | (many diffs) |
| 8 | human | 55064 | SPATA6L | spermatogenesis associated ... | NM_001039395.4 | 38.7% | 21.4% | (many diffs) |
| 9 | human | 55064 | SPATA6L | spermatogenesis associated ... | NM_001353486.2 | 36.4% | 35.4% | (many diffs) |
| 10 | human | 55064 | SPATA6L | spermatogenesis associated ... | XM_024447598.1 | 35.5% | 34.3% | (many diffs) |
| 11 | human | 55064 | SPATA6L | spermatogenesis associated ... | XM_006716800.3 | 31.8% | 31% | (many diffs) |
| 12 | human | 55064 | SPATA6L | spermatogenesis associated ... | XM_011517952.2 | 31.8% | 31% | (many diffs) |
| 13 | human | 55064 | SPATA6L | spermatogenesis associated ... | NM_001353487.1 | 28.3% | 27.3% | (many diffs) |
| 14 | human | 55064 | SPATA6L | spermatogenesis associated ... | XM_024447597.1 | 28.3% | 27.3% | (many diffs) |
| 15 | human | 55064 | SPATA6L | spermatogenesis associated ... | NR_148444.2 | 26.1% | 1_150del;646_1895del | |
| 16 | human | 55064 | SPATA6L | spermatogenesis associated ... | NR_148445.2 | 24.1% | (many diffs) | |
| 17 | human | 55064 | SPATA6L | spermatogenesis associated ... | XR_001746335.2 | 24% | (many diffs) | |
| 18 | human | 55064 | SPATA6L | spermatogenesis associated ... | XR_001746336.1 | 13.7% | (many diffs) | |
| 19 | human | 55064 | SPATA6L | spermatogenesis associated ... | XR_001746338.1 | 13.5% | (many diffs) | |
| 20 | human | 55064 | SPATA6L | spermatogenesis associated ... | XR_001746340.1 | 12.8% | (many diffs) | |
| 21 | human | 55064 | SPATA6L | spermatogenesis associated ... | XR_001746337.1 | 12.3% | (many diffs) | |
| 22 | human | 55064 | SPATA6L | spermatogenesis associated ... | XR_001746341.1 | 12% | (many diffs) | |
| 23 | human | 55064 | SPATA6L | spermatogenesis associated ... | NR_148448.1 | 11.3% | (many diffs) | |
| 24 | human | 55064 | SPATA6L | spermatogenesis associated ... | NR_148447.1 | 10.6% | (many diffs) | |
| 25 | human | 55064 | SPATA6L | spermatogenesis associated ... | NR_148446.2 | 10.6% | (many diffs) | |
| 26 | human | 55064 | SPATA6L | spermatogenesis associated ... | XR_001746339.1 | 8.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 561
- ORF length:
- 495
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc tctggaggtg gtggtggagc tgcagatccg ggcgatttct tgcccaggag 121 tgttcctgcc tggcaaacaa gatgtgtacc tcggggtcta cctcatgaat cagtaccTGG 181 AGACCAACAG CTTTCCCTCT GCGTTCCCCA TTATGATTCA GGAGAGCATG AGATTTGAAA 241 AGGTATTTGA AAGTGCAGTA GATCCTGGAG CTGTAGTAGA CCTTTTGGAA AGCTTCCTAG 301 CCAGGTTTGA ATTAATACAG CTAGTTCCTC CAGTGTGGGA TGAGTTGGCC TACTACGAAG 361 AAAACACACG AGATTTTCTT TTCCCAGAGC CCAAGCTGAC ACCTTCGCAC CCTAGGAGGT 421 GTAGGGAGGT GCTCATGAAG ACGGCTCTGG GTTTTCCAGG CATTGCTCCC AAAATAGAGT 481 TTTCTACAAG GACAGCCATC AGAGAATGTG TGTTTCTGCA TAGAAACAGA TTTCTTGTTG 541 ATTCCAAATC TTGGCTATTG TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 601 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 661 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACCTT TACTTAGGCA 721 GGTTAATAGA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt