Construct: ORF TRCN0000491767
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011055.2_s317c1
- Derived from:
- ccsbBroadEn_01254
- DNA Barcode:
- AGACTACTTGCGATTAGAGAGTGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PPM1A (5494)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491767
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5494 | PPM1A | protein phosphatase, Mg2+/M... | NM_021003.5 | 100% | 100% | |
| 2 | human | 5494 | PPM1A | protein phosphatase, Mg2+/M... | XM_005267779.1 | 100% | 100% | |
| 3 | human | 5494 | PPM1A | protein phosphatase, Mg2+/M... | XM_005267781.1 | 100% | 100% | |
| 4 | human | 5494 | PPM1A | protein phosphatase, Mg2+/M... | XM_011536878.1 | 100% | 100% | |
| 5 | human | 5494 | PPM1A | protein phosphatase, Mg2+/M... | XM_011536879.3 | 100% | 100% | |
| 6 | human | 5494 | PPM1A | protein phosphatase, Mg2+/M... | XM_011536880.3 | 100% | 100% | |
| 7 | human | 5494 | PPM1A | protein phosphatase, Mg2+/M... | XM_011536881.3 | 100% | 100% | |
| 8 | human | 5494 | PPM1A | protein phosphatase, Mg2+/M... | XM_011536882.2 | 100% | 100% | |
| 9 | human | 5494 | PPM1A | protein phosphatase, Mg2+/M... | XM_011536883.1 | 100% | 100% | |
| 10 | human | 5494 | PPM1A | protein phosphatase, Mg2+/M... | XM_017021384.1 | 100% | 100% | |
| 11 | human | 5494 | PPM1A | protein phosphatase, Mg2+/M... | XM_017021385.1 | 100% | 100% | |
| 12 | human | 5494 | PPM1A | protein phosphatase, Mg2+/M... | XM_017021386.1 | 100% | 100% | |
| 13 | human | 5494 | PPM1A | protein phosphatase, Mg2+/M... | XM_017021387.1 | 100% | 100% | |
| 14 | human | 5494 | PPM1A | protein phosphatase, Mg2+/M... | XM_024449637.1 | 100% | 100% | |
| 15 | human | 5494 | PPM1A | protein phosphatase, Mg2+/M... | XM_017021383.1 | 93.6% | 93.6% | 1_78del |
| 16 | human | 5494 | PPM1A | protein phosphatase, Mg2+/M... | XM_011536872.1 | 87.6% | 87.6% | 1_162del |
| 17 | human | 5494 | PPM1A | protein phosphatase, Mg2+/M... | XM_011536876.1 | 87.6% | 87.6% | 1_162del |
| 18 | human | 5494 | PPM1A | protein phosphatase, Mg2+/M... | XM_011536877.1 | 87.6% | 87.6% | 1_162del |
| 19 | human | 5494 | PPM1A | protein phosphatase, Mg2+/M... | XM_017021382.1 | 87.6% | 87.6% | 1_162del |
| 20 | human | 5494 | PPM1A | protein phosphatase, Mg2+/M... | NM_177952.3 | 83.9% | 83.9% | 1_219del |
| 21 | human | 5494 | PPM1A | protein phosphatase, Mg2+/M... | NM_177951.3 | 83.4% | 83% | (many diffs) |
| 22 | human | 5494 | PPM1A | protein phosphatase, Mg2+/M... | XM_017021381.1 | 83.2% | 83.2% | 1_231del |
| 23 | mouse | 19042 | Ppm1a | protein phosphatase 1A, mag... | NM_008910.3 | 93.5% | 98.1% | (many diffs) |
| 24 | mouse | 19042 | Ppm1a | protein phosphatase 1A, mag... | XM_006515603.3 | 93.5% | 98.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1212
- ORF length:
- 1146
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg agcattttta gacaagccaa agatggaaaa gcataatgcc caggggcagg 121 gtaatgggtt gcgatatggg ctaagcagca tgcaaggctg gcgtgttgaa atggaggatg 181 cacatacggc tgtgatcggt ttgccaagtg gacttgaatc gtggtcattc tttgctgtgt 241 atgatgggca tgctggttct caggttgcca aatactgctg tgagcatttg ttagatcaca 301 tcaccaataa ccaggatttt aaagggtctg caggagcacc ttctgtggaa aatgtaaaga 361 atggaatcag aacaggtttt ctggagattg atgaacacat gagagttatg tcagagaaga 421 aacatggtgc agatagaagt gggtcaacag ctgtaggtgt cttaatttct ccccaacata 481 cttatttcat taactgtgga gactcaagag gtttactttg taggaacagg aaagttcatt 541 tcttcacaca agatcacaaa ccaagtaatc cgctggagaa agaacgaatt cagaatgcag 601 gtggctctgt aatgattcag cgtgtgaatg gctctctggc tgtatcgagg gcccttgggg 661 attttgatta caaatgtgtc catggaaaag gtcctactga gcagcttgtc tcaccagagc 721 ctgaagtcca tgatattgaa agatctgaag aagatgatca gttcattatc cttgcatgtg 781 atggtatctg ggatgttatg ggaaatgaag agctctgtga ttttgtaaga tccagacttG 841 AAGTCACTGA TGACCTTGAG AAAGTTTGCA ATGAAGTAGT CGACACCTGT TTGTATAAGG 901 GAAGTCGAGA CAACATGAGT GTGATTTTGA TCTGTTTTCC AAATGCACCC AAAGTATCGC 961 CAGAAGCAGT GAAGAAGGAG GCAGAGTTGG ACAAGTACCT GGAATGCAGA GTAGAAGAAA 1021 TCATAAAGAA GCAGGGGGAA GGCGTCCCCG ACTTAGTCCA TGTGATGCGC ACATTAGCGA 1081 GTGAGAACAT CCCCAGCCTC CCACCAGGGG GTGAATTGGC AAGCAAGAGG AATGTTATTG 1141 AAGCCGTTTA CAATAGACTG AATCCTTACA AAAATGACGA CACTGACTCT ACATCAACAG 1201 ATGATATGTG GTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1261 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1321 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAAGA CTACTTGCGA TTAGAGAGTG 1381 CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t