Construct: ORF TRCN0000491768
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002826.1_s317c1
- Derived from:
- ccsbBroadEn_07640
- DNA Barcode:
- TCCCGATGAGGGCGGCGAGCAATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MRPL28 (10573)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491768
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10573 | MRPL28 | mitochondrial ribosomal pro... | NM_006428.5 | 99.8% | 100% | 417T>C |
| 2 | human | 10573 | MRPL28 | mitochondrial ribosomal pro... | XM_005255041.2 | 99.8% | 100% | 417T>C |
| 3 | human | 10573 | MRPL28 | mitochondrial ribosomal pro... | XM_011522351.2 | 99.8% | 100% | 417T>C |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 834
- ORF length:
- 768
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc tctacacaag tatcccgtgt ggctctggaa gcggctgcag ctgcgggagg 121 gcatctgttc ccgcctgccc ggccactacc tgcgctccct ggaggaggag cggacgccca 181 ctcccgtgca ctataggcct catggggcca agttcaagat caaccccaag aacgggcagc 241 gggagcgtgt ggaggacgtg cccattccca tctactttcc ccccgaatcc cagcgggggt 301 tgtggggcgg cgagggctgg atcctgggcc aaatatatgc caacaacgac aagctctcca 361 agaggctgaa gaaagtgtgg aagccacagc tgtttgagcg agagttctac agtgagatcc 421 tggacaagaa gttcacagtg actgtgacca tgcggaccct ggacctcatc gatgaggctt 481 acgggctcga cttttacaTC CTCAAGACCC CGAAGGAGGA CCTGTGCTCC AAGTTTGGGA 541 TGGACCTGAA GCGAGGGATG CTGCTGCGGC TTGCCCGGCA GGACCCCCAG CTGCACCCCG 601 AGGACCCCGA GCGGCGGGCA GCCATCTACG ACAAGTACAA GGAATTTGCC ATCCCAGAGG 661 AGGAGGCAGA GTGGGTGGGC CTCACGCTGG AGGAGGCCAT TGAGAAGCAG AGACTTTTGG 721 AGGAGAAGGA CCCTGTACCC CTGTTCAAGA TCTATGTGGC GGAGCTGATC CAGCAGCTGC 781 AGCAGCAGGC ACTGTCAGAG CCGGCGGTGG TGCAGAAGAG AGCCAGTGGC CAGTGCCCAA 841 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 901 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 961 TATCTTGTGG AAAGGACGAT CCCGATGAGG GCGGCGAGCA ATAACGCGTT AAGTCgacaa 1021 tcaacctctg gattacaaaa tttgtgaaag att