Construct: ORF TRCN0000491784
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016201.1_s317c1
- Derived from:
- ccsbBroadEn_12434
- DNA Barcode:
- CAAGTCACCGCTGTGGCTCATGAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UBE2O (63893)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491784
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 63893 | UBE2O | ubiquitin conjugating enzym... | XM_017024935.1 | 58.4% | 58.4% | 1_1578del |
| 2 | human | 63893 | UBE2O | ubiquitin conjugating enzym... | NM_022066.4 | 57.3% | 57.3% | 1_1653del |
| 3 | human | 63893 | UBE2O | ubiquitin conjugating enzym... | XM_005257567.1 | 57.3% | 57.3% | 1_1653del;2024_2026delAGC |
| 4 | human | 63893 | UBE2O | ubiquitin conjugating enzym... | XM_024450867.1 | 56.8% | 56.8% | 1_1686del;2057_2059delAGC |
| 5 | human | 63893 | UBE2O | ubiquitin conjugating enzym... | XM_024450866.1 | 55.7% | 55.7% | 1_1761del |
| 6 | human | 63893 | UBE2O | ubiquitin conjugating enzym... | XM_024450865.1 | 55.7% | 55.7% | 1_1761del;2132_2134delAGC |
| 7 | mouse | 217342 | Ube2o | ubiquitin-conjugating enzym... | XM_011248960.1 | 86.5% | 86.1% | (many diffs) |
| 8 | mouse | 217342 | Ube2o | ubiquitin-conjugating enzym... | XM_006533106.1 | 51.6% | 55.3% | (many diffs) |
| 9 | mouse | 217342 | Ube2o | ubiquitin-conjugating enzym... | NM_173755.3 | 50.6% | 54.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2289
- ORF length:
- 2223
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac ctcagccgac gtgatgtggc aggatggctc cgtggaatgc aacatccgct 121 ccaacgacct cttccctgtg caccacctgg acaacaacga gttctgccct ggagacttcg 181 tggtagataa gcgagtccag agctgtccag accctgctgt ctacggtgtg gtacagtctg 241 gggaccacat cggccgtacc tgcatggtga agtggttcaa gctgaggccg agtggggacg 301 acgtggagct gattggagaa gaggaagatg tgagtgttta cgacattgct gaccaccctg 361 actttaggtt ccgtacaact gacatcgtca tccgcatcgg caatactgag gatggggctc 421 ctcacaagga ggatgagcca tcggtgggcc aggtggcccg tgtggacgtc agcagcaagg 481 tggaggtggt gtgggctgac aactcaaaga ccatcatcct gccccagcac ttgtacaaca 541 tagagtctga gattgaggag tcagactacg attcggtaga aggcagcacc agcggggcat 601 cctcggatga atgggaagat gatagtgaca gctgggagac ggacaatggg ctggtggagg 661 acgagcaccc caagatagag gagcccccca tcccacccct ggagcagccg gtggcccctg 721 aggacaaggg agtggtgatc agtgaagagg cagccacagc tgccgtccag ggggctgtgg 781 ccatggctgc ccccatggcc gggctgatgg agaaggctgg caaggacggg ccacccaaga 841 gcttccggga gttgaaagag gccatcaaga tcctggagag cctcaagaac atgactgtgg 901 agcagctgct gacgggctcg cccacctctc cgactgtgga gcctgagaag ccaactcggg 961 agaagaagtt tctggatgac atcaagaagc tacaggaaaa cctcaagaag accctggaca 1021 atgtggccat tgtagaggag gagaagatgg aagcagtgcc cgacgtagag cgcaaggagg 1081 acaagcccga ggggcagtca cctgtgaagg ctgagtggcc cagcgaaacc ccggtgctgt 1141 gccagcagtg tggcggcaag cctggcgtca ccttcaccag cgccaagggc gaggtcttct 1201 ccgtactgga gtttgcaccc tcaaatcatt cttttaagaa aattgagttc cagcctccag 1261 aagccaagaa gttcttcagc acagtgcgga aggagatggc gctgctggct acctcactgc 1321 ctgagggcat catggtcaag acttttgaag atagaatgga cctcttctca gctctcatca 1381 agggccccac tcgaaccccc tacgaggatg gcctctactt gtttgacatc cagctcccca 1441 acatctaccc agccgtgccc ccccacttct gctacctctc ccaatgcagt ggccgcctga 1501 accccaacct gtatgacaat gggaaggtgt gtgtcagcct cctgggcacc tggattggaa 1561 aggggacaga gaggtggaca agcaagtcca gccttctcca ggtgctcatc tccatccaag 1621 gtctgatcct ggtaaatgaa ccatactaca acgaagccgg cttcgacagt gaccgaggcc 1681 tgcaggaagg ctatgaaaac agtcgctgtt acaatgagat ggcgctgatc cgcgtggtgc 1741 agtccatgac ccagctggtg cggcggcccc ccgaggtctt tgagcaggag atcaggcaac 1801 actttagcac tggtggctgg cggctggtga accgtatcga gtcctggctg gaaacccatg 1861 ccctgctgga gaaggcccag gcactgccca acggggtgcc caaggccagc agctcgccag 1921 agcccccagc tGTAGCCGAG CTGTCAGACT CCGGCCAACA AGAACCTGAG GATGGAGGGC 1981 CAGCCCCAGG AGAGGCCTCC CAGGGCTCAG ACTCAGAGGG CGGTGCCCAG GGCCTGGCCT 2041 CAGCTAGCAG GGACCACACA GACCAGACTT CGGAGACCGC ACCAGACGCA TCGGTGCCAC 2101 CCAGTGTGAA ACCAAAGAAG CGGAGAAAGA GCTACCGGAG CTTCTTACCT GAGAAGAGTG 2161 GCTACCCTGA CATCGGCTTC CCCCTCTTCC CACTTTCCAA GGGTTTCATC AAGAGCATCC 2221 GGGGTGTCCT GACGCAGTTC CGGGCTGCCC TGCTAGAGGC AGGCATGCCG GAGTGCACAG 2281 AGGACAAGTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 2341 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 2401 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACAAGTC ACCGCTGTGG CTCATGATAC 2461 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt