Construct: ORF TRCN0000491791
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011634.1_s317c1
- Derived from:
- ccsbBroadEn_11415
- DNA Barcode:
- ACCCCGACGTTAGTAAGTAGCTGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PTDSS1 (9791)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491791
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9791 | PTDSS1 | phosphatidylserine synthase 1 | NM_001290225.2 | 100% | 100% | |
| 2 | human | 9791 | PTDSS1 | phosphatidylserine synthase 1 | NM_014754.3 | 69.1% | 69.1% | 1_438del |
| 3 | mouse | 19210 | Ptdss1 | phosphatidylserine synthase 1 | NM_008959.3 | 62.5% | 67% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1047
- ORF length:
- 981
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gtatgctgtg aactgccatg tgatcacctg ggagaggatt atcagccact 121 ttgatatttt tgcatttgga catttctggg gctgggccat gaaggccttg ctgatccgta 181 gttacggtct ctgctggaca atcagtatta cctgggagct gactgagctc ttcttcatgc 241 atctcctccc caattttgcc gagtgctggt gggatcaagt cattctggac atcctgttgt 301 gcaatggcgg tggcatttgg ctgggcatgg tcgtttgccg gtttttagag atgaggactt 361 accactgggc aagcttcaag gacattcata ccaccaccgg gaagatcaag agagctgttc 421 tgcagttcac tcctgctagc tggacctatg ttcgatggtt tgaccccaaa tcttcttttc 481 agagagtagc tggagtgtac cttttcatga tcatctggca gctgactgag ttgaatacct 541 tcttcttgaa gcatatcttt gtgttccaag ccagtcatcc attaagttgg ggtagaattc 601 tctttattgg tggcatcaca gctcccacag tgagacagta ctacgcttac ctcaccgaca 661 cacagtgcaa gcgcgtagga acacaatgct gggtgtttGG GGTCATTGGT TTCCTGGAGG 721 CCATTGTTTG CATAAAATTT GGACAAGATC TCTTCTCTAA GACCCAAATA CTCTATGTTG 781 TGCTTTGGCT TCTTTGCGTG GCTTTCACCA CTTTCCTCTG TCTGTACGGC ATGATTTGGT 841 ATGCAGAACA CTATGGTCAC CGAGAAAAGA CCTACTCGGA GTGTGAAGAT GGCACCTACA 901 GTCCAGAGAT CTCCTGGCAT CACAGGAAAG GGACAAAAGG TTCTGAAGAC AGCCCACCCA 961 AGCATGCAGG CAACAACGAA AGCCATTCTT CCAGGAGAAG GAATCGGCAT TCCAAGTCAA 1021 AAGTCACCAA TGGCGTTGGA AAGAAATGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 1081 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1141 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAACCCCGAC 1201 GTTAGTAAGT AGCTGGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1261 aagatt