Construct: ORF TRCN0000491825
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021254.2_s317c1
- DNA Barcode:
- ACTTACATCTCACATCAAAAATCC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- NOTCH2NLA (388677)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491825
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 100996717 | NOTCH2NLC | notch 2 N-terminal like C | NM_001364012.2 | 99.7% | 99.5% | 285A>G;473C>T |
| 2 | human | 388677 | NOTCH2NLA | notch 2 N-terminal like A | NM_203458.4 | 99.7% | 99.5% | 285A>G;473C>T |
| 3 | human | 100996763 | NOTCH2NLB | notch 2 N-terminal like B | NM_001364008.1 | 94.5% | 94.7% | 1_39del;228A>G;324A>G |
| 4 | human | 388677 | NOTCH2NLA | notch 2 N-terminal like A | NM_001364006.1 | 94.5% | 94.3% | 1_39del;324A>G;512C>T |
| 5 | human | 100996763 | NOTCH2NLB | notch 2 N-terminal like B | NM_001364007.2 | 85.5% | 85.8% | 1_117del;306A>G;402A>G |
| 6 | human | 100996717 | NOTCH2NLC | notch 2 N-terminal like C | NM_001364013.1 | 80.3% | 80.2% | 1_171del;456A>G;644C>T |
| 7 | human | 100996763 | NOTCH2NLB | notch 2 N-terminal like B | XM_006711700.4 | 78.1% | 78.4% | 1_195del;384A>G;480A>G |
| 8 | human | 101929796 | NOTCH2NLR | notch 2 N-terminal like R | NR_157049.1 | 61.6% | (many diffs) | |
| 9 | human | 100996763 | NOTCH2NLB | notch 2 N-terminal like B | XM_011510256.2 | 57.1% | 57.2% | 1_528del;717A>G;813A>G |
| 10 | human | 100996763 | NOTCH2NLB | notch 2 N-terminal like B | XM_024452030.1 | 51.5% | 51.8% | (many diffs) |
| 11 | human | 100996763 | NOTCH2NLB | notch 2 N-terminal like B | XM_024452031.1 | 51.5% | 51.8% | (many diffs) |
| 12 | human | 100996763 | NOTCH2NLB | notch 2 N-terminal like B | XM_024452032.1 | 51.5% | 51.8% | (many diffs) |
| 13 | human | 100996763 | NOTCH2NLB | notch 2 N-terminal like B | XM_024452035.1 | 51.5% | 51.8% | (many diffs) |
| 14 | human | 4853 | NOTCH2 | notch receptor 2 | NM_001200001.1 | 18.2% | 17.2% | (many diffs) |
| 15 | human | 4853 | NOTCH2 | notch receptor 2 | NM_024408.4 | 9.1% | 8.6% | (many diffs) |
| 16 | mouse | 18129 | Notch2 | notch 2 | NM_010928.2 | 8% | 7.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 780
- ORF length:
- 708
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgtgtgtt acctaccaca atggcacagg atactgcaaa tgtccagaag 121 gcttcttggg ggaatattgt caacatcgag acccctgtga gaagaaccgc tgccagaatg 181 gtgggacttg tgtggcccag gccatgctgg ggaaagccac gtgccgatgt gcctcagggt 241 ttacaggaga ggactgccag tactcgacat ctcatccatg ctttgtgtct cgaccttgcc 301 tgaatggcgg cacatgccat atgctcagcc gggataccta tgagtgcacc tgtcaggtcg 361 ggtttacagg taaggagtgc caatggaccg atgcctgcct gtctcatccc tgtgcaaatg 421 gaagtacctg taccactgtg gccaaccagt tcTCCTGCAA ATGCCTCACA GGCTTCACAG 481 GGCAGAAGTG TGAGACTGAT GTCAATGAGT GTGACATTCC AGGACACTGC CAGCATGGTG 541 GCATCTGCCT CAACCTGCCT GGTTCCTACC AGTGCCAGTG CCTTCAGGGC TTCACAGGCC 601 AGTACTGTGA CAGCCTGTAT GTGCCCTGTG CACCCTCGCC TTGTGTCAAT GGAGGCACCT 661 GTCGGCAGAC TGGTGACTTC ACTTTTGAGT GCAACTGCCT TCCAGAAACA GTGAGAAGAG 721 GAACAGAGCT CTGGGAAAGA GACAGGGAAG TCTGGAATGG AAAAGAACAC GATGAGAATT 781 AGAACCCAGC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 841 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 901 GGCTTTATAT ATCTTGTGGA AAGGACGAAC TTACATCTCA CATCAAAAAT CCACGCGTTA 961 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt