Construct: ORF TRCN0000491884
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006367.1_s317c1
- Derived from:
- ccsbBroadEn_13108
- DNA Barcode:
- AGTCAAACCGGAGGCCTCGTATCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KIF19 (124602)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491884
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 124602 | KIF19 | kinesin family member 19 | XR_001752425.1 | 14.2% | (many diffs) | |
| 2 | human | 124602 | KIF19 | kinesin family member 19 | XR_001752426.1 | 13.8% | (many diffs) | |
| 3 | human | 124602 | KIF19 | kinesin family member 19 | XM_017024158.1 | 13.1% | 3.9% | 1_112del;470_2721del |
| 4 | human | 124602 | KIF19 | kinesin family member 19 | XM_017024157.1 | 13.1% | 3.8% | 1_112del;470_2724del |
| 5 | human | 124602 | KIF19 | kinesin family member 19 | XM_017024156.1 | 12.5% | 3.7% | (many diffs) |
| 6 | human | 124602 | KIF19 | kinesin family member 19 | XM_017024155.1 | 12.4% | 3.7% | (many diffs) |
| 7 | human | 124602 | KIF19 | kinesin family member 19 | XM_017024154.1 | 12.4% | 3.7% | 1_112del;470_2868del |
| 8 | human | 124602 | KIF19 | kinesin family member 19 | XM_005257030.1 | 12.4% | 3.6% | 1_112del;470_2871del |
| 9 | human | 124602 | KIF19 | kinesin family member 19 | XM_017024153.1 | 12.2% | 3.6% | 1_112del;470_2907del |
| 10 | human | 124602 | KIF19 | kinesin family member 19 | XM_017024152.1 | 12.2% | 3.6% | 1_112del;470_2910del |
| 11 | human | 124602 | KIF19 | kinesin family member 19 | NM_153209.4 | 11.8% | 3.5% | (many diffs) |
| 12 | human | 124602 | KIF19 | kinesin family member 19 | XM_005257029.1 | 11.8% | 3.5% | (many diffs) |
| 13 | human | 124602 | KIF19 | kinesin family member 19 | XM_017024151.1 | 11.7% | 3.5% | (many diffs) |
| 14 | human | 124602 | KIF19 | kinesin family member 19 | XM_017024150.1 | 11.7% | 3.5% | (many diffs) |
| 15 | mouse | 286942 | Kif19a | kinesin family member 19A | NM_001102615.1 | 10.9% | 2.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 81
- ORF end:
- 438
- ORF length:
- 357
- Sequence:
-
1 GGTTCAAAGT TTTTTTCTTC CATTTCAGGT GTCGTGAGGC TAGCATCGAT TGATCAACAA 61 GTTTGTACAA AAAAGTTGGC ATGAGCAGAT GGTGGTTCTC ATGGACCCAA TGGAGGATCC 121 CGACGACATC CTGCGGGCGC ATCGCTCCCG GGAGAAGTCC TACCTGTTCG ACGTGGCCTT 181 TGACTTCACC GCCACCCAGG AGATGGTGTA TCAGGCCACC ACCAAGAGCC TCATCGAGGG 241 CGTCATCTCA GGCTACAATG CCACTGTCTT TGCCTATGGC CCCACAGGCT GTGGGAAAAC 301 CTACACCATG CTGGGCACAG ACCAGGAGCC TGGCATCTAT GTTCAGACCC TCAACGACCT 361 CTTCCGTGCC ATCGAGGAGA CCAGCAATGA CATGGAGTAT GAGGTCTCCA TGTCCTACCT 421 GGAGATCATG CAGCTGCTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 481 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 541 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAAGTCAAA CCGGAGGCCT 601 CGTATCAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt