Construct: ORF TRCN0000491902
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001415.1_s317c1
- Derived from:
- ccsbBroadEn_11944
- DNA Barcode:
- TCAATGCCATAAGGCCAACAGTCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CRYL1 (51084)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491902
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51084 | CRYL1 | crystallin lambda 1 | NM_015974.3 | 93.1% | 93.1% | 1_66del |
| 2 | human | 51084 | CRYL1 | crystallin lambda 1 | XM_017020624.1 | 78% | 77.1% | (many diffs) |
| 3 | human | 51084 | CRYL1 | crystallin lambda 1 | NM_001363647.1 | 76.1% | 76.1% | 1_66del;276_277ins162 |
| 4 | human | 51084 | CRYL1 | crystallin lambda 1 | XM_005266416.5 | 59.9% | 59.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 960
- ORF length:
- 891
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gctgtttgcc agtggaggct tccaggtgaa actctatgac attgagcaac 121 agcagataag gaacgccctg gaaaacatca gaaaggagat gaagttgctg gagcaggcag 181 gttctctgaa aggctccctg agtgtggaag agcagctgtc actcatcagt ggttgtccca 241 atatccaaga agcagtagag ggtgccatgc acattcagga atgtgttcca gaagatctag 301 aactgaagaa gaagattttt gctcagttag attccatcat tgatgatcga gtgatcttaa 361 gcagttccac ttcttgtctc atgccttcca agttgtttgc tggcttggtc catgtgaagc 421 aatgcatcgt ggctcatcct gtgaatccgc catactacat cccgctggtt gagctggtcc 481 cccacccgga gacggcccct acgacagtgg acagaaccca cgccctgatg aagaagattg 541 gacagtgccc catgcgagtc cagaaggagg tggccggcTT CGTTCTGAAC CGCCTGCAAT 601 ATGCAATCAT CAGCGAGGCC TGGCGGCTAG TGGAGGAAGG AATCGTGTCT CCTAGTGACC 661 TGGACCTTGT CATGTCAGAA GGGTTGGGCA TGCGGTATGC ATTCATTGGA CCCCTGGAAA 721 CCATGCATCT CAATGCAGAA GGTATGTTAA GCTACTGCGA CAGATACAGC GAAGGCATAA 781 AACATGTCCT ACAGACTTTT GGACCCATTC CAGAGTTTTC CAGGGCCACT GCTGAGAAGG 841 TTAACCAGGA CATGTGCATG AAGGTCCCTG ATGACCCGGA GCACTTAGCT GCCAGGAGGC 901 AGTGGAGGGA CGAGTGCCTC ATGAGACTCG CCAAGTTGAA GAGTCAAGTG CAGCCCCAGT 961 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1021 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1081 TTTATATATC TTGTGGAAAG GACGATCAAT GCCATAAGGC CAACAGTCCA CGCGTTAAGT 1141 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt