Transcript: Human NM_015974.3

Homo sapiens crystallin lambda 1 (CRYL1), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-08-07
Taxon:
Homo sapiens (human)
Gene:
CRYL1 (51084)
Length:
1483
CDS:
64..1023

Additional Resources:

NCBI RefSeq record:
NM_015974.3
NBCI Gene record:
CRYL1 (51084)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_015974.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000422569 GCATGCGGTATGCATTCATTG pLKO_005 749 CDS 100% 10.800 15.120 N CRYL1 n/a
2 TRCN0000444155 GCCCGTCACTTTGGATCATAG pLKO_005 1183 3UTR 100% 10.800 15.120 N CRYL1 n/a
3 TRCN0000419316 GGAACACTGCAAGCCCTTAAT pLKO_005 1071 3UTR 100% 13.200 9.240 N CRYL1 n/a
4 TRCN0000064940 CCAGGTGAAACTCTATGACAT pLKO.1 153 CDS 100% 4.950 3.465 N CRYL1 n/a
5 TRCN0000064939 GCATCTCAATGCAGAAGGTAT pLKO.1 786 CDS 100% 4.950 3.465 N CRYL1 n/a
6 TRCN0000064942 GCCTGCAATATGCAATCATCA pLKO.1 653 CDS 100% 4.950 3.465 N CRYL1 n/a
7 TRCN0000064941 CCCAATATCCAAGAAGCAGTA pLKO.1 298 CDS 100% 4.050 2.835 N CRYL1 n/a
8 TRCN0000064938 CCACTTCTTGTCTCATGCCTT pLKO.1 428 CDS 100% 2.640 1.848 N CRYL1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_015974.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11944 pDONR223 100% 93.1% 93.1% None 1_66del n/a
2 ccsbBroad304_11944 pLX_304 0% 93.1% 93.1% V5 1_66del n/a
3 TRCN0000491902 TCAATGCCATAAGGCCAACAGTCC pLX_317 42.8% 93.1% 93.1% V5 1_66del n/a
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