Construct: ORF TRCN0000491912
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021146.2_s317c1
- DNA Barcode:
- GTGAGCGCCCATTGACCCGGGAAT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- BDKRB2 (624)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491912
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 624 | BDKRB2 | bradykinin receptor B2 | NM_000623.3 | 99.9% | 100% | 792A>G |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1245
- ORF length:
- 1173
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgttctct ccctggaaga tatcaatgtt tctgtctgtt cgtgaggact 121 ccgtgcccac cacggcctct ttcagcgccg acatgctcaa tgtcaccttg caagggccca 181 ctcttaacgg gacctttgcc cagagcaaat gcccccaagt ggagtggctg ggctggctca 241 acaccatcca gccccccttc ctctgggtgc tgttcgtgct ggccacccta gagaacatct 301 ttgtcctcag cgtcttctgc ctgcacaaga gcagctgcac ggtggcagag atctacctgg 361 ggaacctggc cgcagcagac ctgatcctgg cctgcgggct gcccttctgg gccatcacca 421 tctccaacaa cttcgactgg ctctttgggg agacgctctg ccgcgtggtg aatgccatta 481 tctccatgaa cctgtacagc agcatctgtt tcctgatgct ggtgagcatc gaccgctacc 541 tggccctggt gaaaaccatg tccatgggcc ggatgcgcgg cgtgcgctgg gccaagctct 601 acagcttggt gatctggggg tgtacgctgc tcctgagctc acccatgctg gtgttccgga 661 ccatgaagga gtacagcgat gagggccaca acgtcaccgc ttgtgtcatc agctacccat 721 ccctcatctg ggaagtgttc accaacatgc tcctgaatgt cgtgggcttc ctgctgcccc 781 tgagtgtcat caccttctgc acgatgcaga tcatgcaggt gctgcggaac aacgagatgc 841 agaagttcaa ggagatccag acggagagga gggccacggt gctagtcctg gttgtgctgc 901 tgctattcat catctgctgg ctgcccttcc agatcagcac cttcctGGAT ACGCTGCATC 961 GCCTCGGCAT CCTCTCCAGC TGCCAGGACG AGCGCATCAT CGATGTAATC ACACAGATCG 1021 CCTCCTTCAT GGCCTACAGC AACAGCTGCC TCAACCCACT GGTGTACGTG ATCGTGGGCA 1081 AGCGCTTCCG AAAGAAGTCT TGGGAGGTGT ACCAGGGAGT GTGCCAGAAA GGGGGCTGCA 1141 GGTCAGAACC CATTCAGATG GAGAACTCCA TGGGCACACT GCGGACCTCC ATCTCCGTGG 1201 AACGCCAGAT TCACAAACTG CAGGACTGGG CAGGGAGCAG ACAGTAGAAC CCAGCTTTCT 1261 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1321 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1381 GTGGAAAGGA CGAGTGAGCG CCCATTGACC CGGGAATACG CGTTAAGTCg acaatcaacc 1441 tctggattac aaaatttgtg aaagatt