Construct: ORF TRCN0000491916
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019761.2_s317c1
- DNA Barcode:
- CCAAAACTCCAATTATGGTCAGGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STYK1 (55359)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491916
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55359 | STYK1 | serine/threonine/tyrosine k... | NM_018423.3 | 99.8% | 99.5% | 610A>G;1266_1267insG |
| 2 | human | 55359 | STYK1 | serine/threonine/tyrosine k... | XM_005253417.2 | 99.8% | 99.5% | 610A>G;1266_1267insG |
| 3 | human | 55359 | STYK1 | serine/threonine/tyrosine k... | XM_011520736.1 | 99.8% | 99.5% | 610A>G;1266_1267insG |
| 4 | human | 55359 | STYK1 | serine/threonine/tyrosine k... | XM_011520737.2 | 99.8% | 99.5% | 610A>G;1266_1267insG |
| 5 | human | 55359 | STYK1 | serine/threonine/tyrosine k... | XM_011520738.2 | 99.8% | 99.5% | 610A>G;1266_1267insG |
| 6 | human | 55359 | STYK1 | serine/threonine/tyrosine k... | XM_017019579.1 | 68.5% | 68.3% | 0_1ins396;214A>G;870_871insG |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1338
- ORF length:
- 1269
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gggcatgaca cggatgctcc tggaatgcag tctcagtgac aagttgtgtg 121 tcatccagga gaagcagtat gaagtgatta tcgtcccaac tttgttggtt actatcttcc 181 tcatccttct tggggtcatc ctgtggcttt ttatcagaga acaaagaact caacagcagc 241 gttctggacc tcaaggcatt gcccctgttc ctccacctag ggacctaagc tgggaagcag 301 gacatggagg aaatgtggct ttgccactta aggagacatc cgtggaaaac tttctgggag 361 ctaccacacc tgccctggct aagctgcagg tgccgcggga gcaactctct gaagttctgg 421 agcagatttg cagtggtagc tgtgggccca tctttcgagc caatatgaac actggggacc 481 cttctaagcc caagagtgtt attctcaagg ctttaaaaga accagctggg ctccatgagg 541 tacaagattt cttagggcga atccaattcc atcaatacct ggggaaacac aaaaacctgg 601 tgcagctgga aggctgctgc actgaaaagc tgccactcta tatggtgttg gaggatgtgg 661 cccaggggga cctgctcggc tttctctgga cctgtcggcg ggatgtgatg actatggatg 721 gtcttctcta tgatctcaca gaaaaacaag tatatcacat cggaaagcag gtccttttgg 781 cgctggaatt cctgcaggag aagcatttgt tccatgggga tgtggcagcc aggaatattc 841 tgatgcaaag tgatctcact gctaagctct gtggattagg cctggcttat gaagtttaca 901 cccgaggggc catctcctct actcaaacca tacctctcaa gtggcttgcc ccagaacggc 961 ttctcctgag acctgctagc atcagagcag atgtctggtc ttttggGATC CTGCTCTATG 1021 AGATGGTGAC TCTAGGAGCA CCACCGTATC CTGAAGTCCC TCCTACCAGC ATCCTAGAGC 1081 ATCTCCAAAG AAGGAAAATC ATGAAGAGAC CCAGTAGCTG CACACATACC ATGTACAGTA 1141 TCATGAAGTC CTGCTGGCGC TGGCGTGAGG CTGACCGCCC CTCACCTAGA GAGCTGCGCT 1201 TGCGCCTAGA AGCTGCCATT AAAACTGCAG ATGACGAGGC TGTGTTACAA GTACCAGAGT 1261 TGGTGGTACC TGAACTGTAT GCAGCTGTGG CCGGCATCAG AGTGGAGAGC CTCTTCTACA 1321 ACTATAGCAT GCTTGACCCA GCTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1381 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1441 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CCAAAACTCC AATTATGGTC 1501 AGGGACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt