Construct: ORF TRCN0000491951

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007841.1_s317c1
Derived from:
ccsbBroadEn_05788
DNA Barcode:
GGGGGTACAACGGGGGCATTATCG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
AP2A2 (161)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000491951
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 161 AP2A2 adaptor related protein com... NM_012305.4 99.9% 99.7% 1759A>N;1948A>N
2 human 161 AP2A2 adaptor related protein com... NM_001242837.1 99.8% 99.6% 813_815delAGA;1762A>N;1951A>N
3 human 161 AP2A2 adaptor related protein com... XM_011519929.1 91.7% 91.5% (many diffs)
4 human 161 AP2A2 adaptor related protein com... XM_011519930.3 69.7% 69.7% (many diffs)
5 human 161 AP2A2 adaptor related protein com... NR_144509.2 57.7% (many diffs)
6 human 161 AP2A2 adaptor related protein com... NR_144510.2 57.6% (many diffs)
7 mouse 11772 Ap2a2 adaptor-related protein com... NM_007459.3 87.1% 97.2% (many diffs)
8 mouse 11772 Ap2a2 adaptor-related protein com... XM_006536154.1 87% 97.1% (many diffs)
9 mouse 11772 Ap2a2 adaptor-related protein com... XM_006536155.3 50% 55.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2883
ORF length:
2817
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgcc ggccgtgtcc aagggggacg ggatgcgggg cctggcggtc ttcatctcgg
121atatccgcaa ctgtaaaagt aaagaagcag aaataaaaag gataaacaag gaactggcaa
181atatcagatc aaaatttaaa ggtgacaagg ctcttgatgg ctatagtaaa aaaaagtacg
241tctgcaagtt gctcttcatc tttctccttg gtcatgacat tgactttgga cacatggagg
301ctgtgaacct gctgagttca aacagataca cggaaaagca gatcggctac cttttcatct
361ctgtgttggt gaactcaaac agtgagctga tccgcctgat caacaacgcc atcaagaatg
421acctggccag ccgcaacccc accttcatgg gcctggccct gcactgcatc gccagcgtgg
481gcagccggga gatggccgag gccttcgccg gggagatccc taaggtcctc gtagccggag
541acactatgga cagcgtgaag cagagcgcgg ccctgtgctt gctgcgcctg tacaggacgt
601cccccgatct tgtccccatg ggcgactgga catcccgagt ggtgcacctg ctcaatgacc
661agcacttggg tgtggtaact gcagccacaa gtctgatcac cactttagca cagaagaacc
721cagaagagtt taaaacctcc gtgtctctgg ctgtctctag gctaagcaga atcgtgacgt
781ctgcatccac agatctccag gattacactt actattttgt cccggctccc tggctgtctg
841tcaaactgct gagactgctg cagtgctacc cacccccaga ccctgcagtg cgaggccgcc
901tgactgagtg cctggagacc atcctgaaca aagcccaaga accgcccaag tcgaagaagg
961tccagcactc caacgcgaag aatgccgtgc tcttcgaggc catcagctta atcattcacc
1021atgacagtga gccgaacctg ctcgtccgtg cctgcaacca gttgggccag tttctgcagc
1081accgcgagac caacctgcgc tacctggccc tggagagcat gtgcacgctg gccagctctg
1141agttctccca tgaggctgtc aagacgcaca tcgagacggt catcaacgcc ctgaagactg
1201agcgggacgt gagcgtgcgg cagcgggccg tggacctcct ctacgccatg tgcgaccgca
1261gcaacgcccc acagatcgtg gccgagatgc tgagctatct ggagacagct gactactcca
1321tccgagaaga gattgtgctg aaggtcgcca tcctggctga gaagtacgcg gtggactaca
1381cctggtatgt ggataccatc ttgaacttga tccgaattgc tggtgattac gtgagtgaag
1441aggtgtggta ccgagtcatt cagatcgtca tcaaccggga cgacgtgcag ggctacgcgg
1501ccaagactgt gttcgaggct cttcaggctc ccgcgtgcca cgagaacctg gtcaaagtgg
1561gcggctacat cctgggggag tttggaaact tgatagctgg agacccgaga tccagcccgc
1621tgatccagtt ccacctgctg cactccaagt tccacctgtg cagcgtcccc acccgcgcgc
1681tgctcctgtc cacctacatc aagttcgtga acctcttccc ggaggtgaag cccaccatcc
1741aggacgtgct gcgcagcgac agccagctca ggaacgcaga cgtggagctg cagcagcgtg
1801ctgtggagta cctgcggctc agcnccgtgg ccagcaccga cattctggcg accgtgctgg
1861aggagatgcc cccattcccg gagcgggagt cctccatctt ggcaaagctc aagaagaaga
1921agggccccag cacggtgaca gacctggagg acaccaagcg ggacaggagt gtggacgtga
1981acgggggtcc tgagcctgcc ccagccagta ccngcgccgt gtctacgcct tctccgtcgg
2041cagacctgct gggtctcggg gctgcccccc ctgcccccgc gggcccccca ccctcctccg
2101gcggcagcgg gctgctcgtg gacgtgttct cagactcggc ctctgtggtc gcgcctctcg
2161ctcctggctc cgaagacaac tttgccaggt ttgtttgtaa aaacaatggt gtgttgtttg
2221aaaaccagct gcttcaaatt ggacttaagt ctgaatttcg gcagaattta ggtcggatgt
2281ttatctttta tggtaataag acctccacgc agttcctaaa ctttacccca acactaatct
2341gttcagacga ccttcagcct aacctgaacc tgcagaccaa gcccgtggac ccgaccgtgg
2401aggggggcgc gcaggtgcag caggtggtca acatagagtg cgtgtccgac ttcacggagg
2461cgccagtcct caacattcag ttcaggtatg ggggcacctt ccagaacgtg tctgtgcagc
2521tgcccatcac tctcaacaaa ttcttccagc cgacagaaat ggcttctcag gatttctttc
2581aacgttggaa gcagttgagc aatccacagc aggaagtgca gaacatcttc aaagcaaagc
2641acccaatgga cacagaagtc accaaagcca agatcattgg atttggttct gcacttcttg
2701aagaagttga tcctaatcct gcgaatttcg tgggagctgg aatcatccac acgaaaacca
2761cccagattgg atgcctgctg cgcttGGAGC CGAACCTGCA AGCCCAGATG TACCGGCTCA
2821CGCTGCGCAC AAGTAAGGAA GCCGTTTCTC AGAGATTATG TGAATTGCTC TCAGCGCAGT
2881TTTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC
2941TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT
3001GGCTTTATAT ATCTTGTGGA AAGGACGAGG GGGTACAACG GGGGCATTAT CGACGCGTTA
3061AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt

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