Construct: ORF TRCN0000491951
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007841.1_s317c1
- Derived from:
- ccsbBroadEn_05788
- DNA Barcode:
- GGGGGTACAACGGGGGCATTATCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AP2A2 (161)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491951
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 161 | AP2A2 | adaptor related protein com... | NM_012305.4 | 99.9% | 99.7% | 1759A>N;1948A>N |
| 2 | human | 161 | AP2A2 | adaptor related protein com... | NM_001242837.1 | 99.8% | 99.6% | 813_815delAGA;1762A>N;1951A>N |
| 3 | human | 161 | AP2A2 | adaptor related protein com... | XM_011519929.1 | 91.7% | 91.5% | (many diffs) |
| 4 | human | 161 | AP2A2 | adaptor related protein com... | XM_011519930.3 | 69.7% | 69.7% | (many diffs) |
| 5 | human | 161 | AP2A2 | adaptor related protein com... | NR_144509.2 | 57.7% | (many diffs) | |
| 6 | human | 161 | AP2A2 | adaptor related protein com... | NR_144510.2 | 57.6% | (many diffs) | |
| 7 | mouse | 11772 | Ap2a2 | adaptor-related protein com... | NM_007459.3 | 87.1% | 97.2% | (many diffs) |
| 8 | mouse | 11772 | Ap2a2 | adaptor-related protein com... | XM_006536154.1 | 87% | 97.1% | (many diffs) |
| 9 | mouse | 11772 | Ap2a2 | adaptor-related protein com... | XM_006536155.3 | 50% | 55.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2883
- ORF length:
- 2817
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc ggccgtgtcc aagggggacg ggatgcgggg cctggcggtc ttcatctcgg 121 atatccgcaa ctgtaaaagt aaagaagcag aaataaaaag gataaacaag gaactggcaa 181 atatcagatc aaaatttaaa ggtgacaagg ctcttgatgg ctatagtaaa aaaaagtacg 241 tctgcaagtt gctcttcatc tttctccttg gtcatgacat tgactttgga cacatggagg 301 ctgtgaacct gctgagttca aacagataca cggaaaagca gatcggctac cttttcatct 361 ctgtgttggt gaactcaaac agtgagctga tccgcctgat caacaacgcc atcaagaatg 421 acctggccag ccgcaacccc accttcatgg gcctggccct gcactgcatc gccagcgtgg 481 gcagccggga gatggccgag gccttcgccg gggagatccc taaggtcctc gtagccggag 541 acactatgga cagcgtgaag cagagcgcgg ccctgtgctt gctgcgcctg tacaggacgt 601 cccccgatct tgtccccatg ggcgactgga catcccgagt ggtgcacctg ctcaatgacc 661 agcacttggg tgtggtaact gcagccacaa gtctgatcac cactttagca cagaagaacc 721 cagaagagtt taaaacctcc gtgtctctgg ctgtctctag gctaagcaga atcgtgacgt 781 ctgcatccac agatctccag gattacactt actattttgt cccggctccc tggctgtctg 841 tcaaactgct gagactgctg cagtgctacc cacccccaga ccctgcagtg cgaggccgcc 901 tgactgagtg cctggagacc atcctgaaca aagcccaaga accgcccaag tcgaagaagg 961 tccagcactc caacgcgaag aatgccgtgc tcttcgaggc catcagctta atcattcacc 1021 atgacagtga gccgaacctg ctcgtccgtg cctgcaacca gttgggccag tttctgcagc 1081 accgcgagac caacctgcgc tacctggccc tggagagcat gtgcacgctg gccagctctg 1141 agttctccca tgaggctgtc aagacgcaca tcgagacggt catcaacgcc ctgaagactg 1201 agcgggacgt gagcgtgcgg cagcgggccg tggacctcct ctacgccatg tgcgaccgca 1261 gcaacgcccc acagatcgtg gccgagatgc tgagctatct ggagacagct gactactcca 1321 tccgagaaga gattgtgctg aaggtcgcca tcctggctga gaagtacgcg gtggactaca 1381 cctggtatgt ggataccatc ttgaacttga tccgaattgc tggtgattac gtgagtgaag 1441 aggtgtggta ccgagtcatt cagatcgtca tcaaccggga cgacgtgcag ggctacgcgg 1501 ccaagactgt gttcgaggct cttcaggctc ccgcgtgcca cgagaacctg gtcaaagtgg 1561 gcggctacat cctgggggag tttggaaact tgatagctgg agacccgaga tccagcccgc 1621 tgatccagtt ccacctgctg cactccaagt tccacctgtg cagcgtcccc acccgcgcgc 1681 tgctcctgtc cacctacatc aagttcgtga acctcttccc ggaggtgaag cccaccatcc 1741 aggacgtgct gcgcagcgac agccagctca ggaacgcaga cgtggagctg cagcagcgtg 1801 ctgtggagta cctgcggctc agcnccgtgg ccagcaccga cattctggcg accgtgctgg 1861 aggagatgcc cccattcccg gagcgggagt cctccatctt ggcaaagctc aagaagaaga 1921 agggccccag cacggtgaca gacctggagg acaccaagcg ggacaggagt gtggacgtga 1981 acgggggtcc tgagcctgcc ccagccagta ccngcgccgt gtctacgcct tctccgtcgg 2041 cagacctgct gggtctcggg gctgcccccc ctgcccccgc gggcccccca ccctcctccg 2101 gcggcagcgg gctgctcgtg gacgtgttct cagactcggc ctctgtggtc gcgcctctcg 2161 ctcctggctc cgaagacaac tttgccaggt ttgtttgtaa aaacaatggt gtgttgtttg 2221 aaaaccagct gcttcaaatt ggacttaagt ctgaatttcg gcagaattta ggtcggatgt 2281 ttatctttta tggtaataag acctccacgc agttcctaaa ctttacccca acactaatct 2341 gttcagacga ccttcagcct aacctgaacc tgcagaccaa gcccgtggac ccgaccgtgg 2401 aggggggcgc gcaggtgcag caggtggtca acatagagtg cgtgtccgac ttcacggagg 2461 cgccagtcct caacattcag ttcaggtatg ggggcacctt ccagaacgtg tctgtgcagc 2521 tgcccatcac tctcaacaaa ttcttccagc cgacagaaat ggcttctcag gatttctttc 2581 aacgttggaa gcagttgagc aatccacagc aggaagtgca gaacatcttc aaagcaaagc 2641 acccaatgga cacagaagtc accaaagcca agatcattgg atttggttct gcacttcttg 2701 aagaagttga tcctaatcct gcgaatttcg tgggagctgg aatcatccac acgaaaacca 2761 cccagattgg atgcctgctg cgcttGGAGC CGAACCTGCA AGCCCAGATG TACCGGCTCA 2821 CGCTGCGCAC AAGTAAGGAA GCCGTTTCTC AGAGATTATG TGAATTGCTC TCAGCGCAGT 2881 TTTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 2941 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 3001 GGCTTTATAT ATCTTGTGGA AAGGACGAGG GGGTACAACG GGGGCATTAT CGACGCGTTA 3061 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt