Construct: ORF TRCN0000491969
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006019.2_s317c1
- Derived from:
- ccsbBroadEn_07804
- DNA Barcode:
- CTATTCAATCGCCAATAGCAGCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DNAJC8 (22826)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491969
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 22826 | DNAJC8 | DnaJ heat shock protein fam... | NM_014280.3 | 99.8% | 100% | 552G>A |
| 2 | human | 22826 | DNAJC8 | DnaJ heat shock protein fam... | NR_159454.1 | 40.2% | (many diffs) | |
| 3 | mouse | 68598 | Dnajc8 | DnaJ heat shock protein fam... | NM_172400.3 | 90.9% | 99.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 825
- ORF length:
- 759
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcttcagga gagagcggga cttcaggcgg cggaggcagc accgaggaag 121 catttatgac cttctacagt gaggtgaaac aaatagagaa gagagactcg gttctaactt 181 cgaaaaatca gattgaaaga ctgacccgtc ctggttcctc ttacttcaat ttgaacccat 241 ttgaggttct tcagatagat cctgaagtta cagatgaaga aataaaaaag aggtttcggc 301 agttatccat cttggtgcat cctgacaaaa atcaagatga tgctgacaga gcacaaaagg 361 cttttgaagc tgtggacaaa gcttacaagt tgctactgga tcaggagcaa aagaagaggg 421 ccctggatgt aattcaggca ggaaaagaat acgtggaaca cactgtgaaa gagcgaaaaa 481 aacaattaaa gaaggaagga aaacctacaa ttgtagagga ggatgatcct gagctgttca 541 aacaagctgt atataaacag acaatgaaac tctttgcaga gctggaaatt aaaaggaaag 601 agagagaagc caaagaaatg catgaaagga aacgacaaag ggaagAAGAG ATTGAAGCTC 661 AAGAAAAAGC CAAACGGGAA AGAGAGTGGC AGAAAAACTT TGAGGAAAGT CGAGATGGTC 721 GTGTGGACAG CTGGCGAAAC TTCCAAGCCA ATACGAAGGG GAAGAAAGAG AAGAAAAATC 781 GGACCTTCCT GAGACCACCG AAAGTAAAAA TGGAGCAACG TGAGTGCCCA ACTTTCTTGT 841 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 901 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 961 GAAAGGACGA CTATTCAATC GCCAATAGCA GCTTACGCGT TAAGTCgaca atcaacctct 1021 ggattacaaa atttgtgaaa gatt