Construct: ORF TRCN0000491971
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019789.2_s317c1
- DNA Barcode:
- TTAACCTATTAGATCGGCCGAGGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GALK1 (2584)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491971
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2584 | GALK1 | galactokinase 1 | NM_000154.2 | 99.8% | 99.7% | 189G>A;1176_1177insG |
| 2 | mouse | 14635 | Galk1 | galactokinase 1 | NM_016905.2 | 85.3% | 87.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1248
- ORF length:
- 1179
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggctgctttg agacagcccc aggtcgcgga gctgctggcc gaggcccggc 121 gagccttccg ggaggagttc ggggccgagc ccgagctggc cgtgtcagcg ccgggccgcg 181 tcaacctcat cggggaacac acggactaca accagggcct ggtgctgcct atggctctgg 241 agctcatgac ggtgctagtg ggcagccccc gcaaggatgg gctggtgtct ctcctcacca 301 cctctgaggg tgccgatgag ccccagcggc tgcagtttcc actgcccaca gcccagcgct 361 cgctggagcc tgggactcct cggtgggcca actatgtcaa gggagtgatt cagtactacc 421 cagctgcccc cctccctggc ttcagtgcag tggtggtcag ctcagtgccc ctggggggtg 481 gcctgtccag ctcagcatcc ttggaagtgg ccacgtacac cttcctccag cagctctgtc 541 cagactcggg cacaatagct gcccgcgccc aggtgtgtca gcaggccgag cacagcttcg 601 cagggatgcc ctgtggcatc atggaccagt tcatctcact tatgggacag aaaggccacg 661 cgctgctcat tgactgcagg tccttggaga ccagcctggt gccactctcg gaccccaagc 721 tggccgtgct catcaccaac tctaatgtcc gccactccct ggcctccagc gagtaccctg 781 tgcggcggcg ccaatgtgaa gaagtggccc gggcgctggg caaggaaagc ctccgggagg 841 tacaactgga agagctagag gctgccaggg acctggtgag caaagagggc ttccggcggg 901 cccggcacgt ggtgggggag attcggcgca cggcccaggc agcggccgcc ctgagacgtg 961 gcGACTACAG AGCCTTTGGC CGCCTCATGG TGGAGAGCCA CCGCTCACTC AGAGACGACT 1021 ATGAGGTGAG CTGCCCAGAG CTGGACCAGC TGGTGGAGGC TGCGCTTGCT GTGCCTGGGG 1081 TTTATGGCAG CCGCATGACG GGCGGTGGCT TCGGTGGCTG CACGGTGACA CTGCTGGAGG 1141 CCTCCGCTGC TCCCCACGCC ATGCGGCACA TCCAGGAGCA CTACGGCGGG ACTGCCACCT 1201 TCTACCTCTC TCAAGCAGCC GATGGAGCCA AGGTGCTGTG CTTGGACCCA GCTTTCTTGT 1261 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1321 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1381 GAAAGGACGA TTAACCTATT AGATCGGCCG AGGGACGCGT TAAGTCgaca atcaacctct 1441 ggattacaaa atttgtgaaa gatt