Construct: ORF TRCN0000491980
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010162.1_s317c1
- Derived from:
- ccsbBroadEn_10584
- DNA Barcode:
- GGTTACCCCCCCCTCGCAATCAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TINCR (257000)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491980
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 257000 | TINCR | TINCR ubiquitin domain cont... | NR_027064.3 | 12.1% | 1_522del;835T>C;976_3733del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 522
- ORF length:
- 453
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaaaccctgg cctgaccagc tgatgcacac tgcttcagac actcctgctg 121 gagccccagt ccctgacaag gacctaggac atttttgctc ctgcccagcc tatcgggagg 181 gagccttgag cctttcagct cTGCTGTGTG ACTTTGAGGT TGTTGCTCCC CTCTTGGGGC 241 CCTGGGTGCC CTGTCTTCAG TGGAAAGCAC TGTGCCACCT TGGAAAGCTC CCATGGGCAG 301 CCAGAGGGCA TCGCAAGAAG AGAAGCACAG AAGGGGCAGG AGAGACACTC AGAGGCACTT 361 CCGCTCTTGC CCAGGACATT CTCCCAGCCA CACCTTTGCC CAAGCCGTGC CCCCTGCCTG 421 GAGCACTTTT CAACCTCTTC TCTGCAGCTC CAATACACCT GGGATTGCAG TCTCCTCCAG 481 GAAGTCTTCT CAGATTCCCT CCTTCCCAGC CAGAGAGCAC CTTGCCAACT TTCTTGTACA 541 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 601 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 661 AGGACGAGGT TACCCCCCCC TCGCAATCAA AACGCGTTAA GTCgacaatc aacctctgga 721 ttacaaaatt tgtgaaagat t