Construct: ORF TRCN0000491995
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006035.1_s317c1
- Derived from:
- ccsbBroadEn_00549
- DNA Barcode:
- TTCGATTATTTAGGCATGGCATCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FDX1 (2230)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491995
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2230 | FDX1 | ferredoxin 1 | NM_004109.5 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 618
- ORF length:
- 552
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgccgctggg ggcgcccggc tgctgcgcgc cgcttctgct gtcctcggcg 121 gcccggccgg ccggtggctg caccacgctg ggtcccgcgc tggatccagc ggcctgctga 181 ggaaccgggg gccgggcggg agcgcggagg cgagccggTC GCTGAGCGTG TCGGCGCGGG 241 CCCGGAGCAG CTCAGAAGAT AAAATAACAG TCCACTTTAT AAACCGTGAT GGTGAAACAT 301 TAACAACCAA AGGAAAAGTT GGTGATTCTC TGCTAGATGT TGTGGTTGAA AATAATCTAG 361 ATATTGATGG CTTTGGTGCA TGTGAGGGAA CCCTGGCTTG TTCAACCTGT CACCTCATCT 421 TTGAAGATCA CATATATGAG AAGTTAGATG CAATCACTGA TGAGGAGAAT GACATGCTCG 481 ATCTGGCATA TGGACTAACA GACAGATCAC GGTTGGGCTG CCAAATCTGT TTGACAAAAT 541 CTATGGACAA TATGACTGTT CGAGTGCCTG AAACAGTGGC TGATGCCAGA CAATCCATTG 601 ATGTGGGCAA GACCTCCTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 661 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 721 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATTCGATT ATTTAGGCAT 781 GGCATCTACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt