Construct: ORF TRCN0000492042
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019326.2_s317c1
- DNA Barcode:
- CAATCACCTTTTAATGAAATTTGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GPR34 (2857)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492042
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2857 | GPR34 | G protein-coupled receptor 34 | NM_001097579.2 | 99.8% | 99.7% | 960T>C;1143_1144insG |
| 2 | human | 2857 | GPR34 | G protein-coupled receptor 34 | NM_005300.4 | 99.8% | 99.7% | 960T>C;1143_1144insG |
| 3 | human | 2857 | GPR34 | G protein-coupled receptor 34 | XM_005272597.4 | 99.8% | 99.7% | 960T>C;1143_1144insG |
| 4 | mouse | 23890 | Gpr34 | G protein-coupled receptor 34 | NM_011823.4 | 85.4% | 89.2% | (many diffs) |
| 5 | mouse | 23890 | Gpr34 | G protein-coupled receptor 34 | XM_006527624.3 | 85.4% | 89.2% | (many diffs) |
| 6 | mouse | 23890 | Gpr34 | G protein-coupled receptor 34 | XM_006527625.3 | 85.4% | 89.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1221
- ORF length:
- 1146
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatgaga agtcatacca taacaatgac gacaacttca gtcagcagct 121 ggccttactc ctcccacaga atgcgcttta taaccaatca tagcgaccaa ccgccacaaa 181 acttctcagc aacaccaaat gttactacct gtcccatgga tgaaaaattg ctatctactg 241 tgttaaccac atcctactct gttattttca tcgtgggact ggttgggaac ataatcgccc 301 tctatgtatt tctgggtatt caccgtaaaa gaaattccat tcaaatttat ctacttaacg 361 tagccattgc agacctccta ctcatcttct gcctcccttt ccgaataatg tatcatatta 421 accaaaacaa gtggacacta ggtgtgattc tgtgcaaggt tgtgggaaca ctgttttata 481 tgaacatgta cattagcatt attttgcttg gattcatcag tttggatcgc tatataaaaa 541 ttaatcggtc tatacagcaa cggaaggcaa taacaaccaa acaaagtatt tatgtctgtt 601 gtatagtatg gatgcttgct cttggtggat tcctaactat gattatttta acacttaaga 661 aaggagggca taattccaca atgtgtttcc attacagaga taagcataac gcaaaaggag 721 aagccatttt taacttcatt cttgtggtaa tgttctggct aattttctta ctaataatcc 781 tttcatatat taagattggg aagaatctat tgaggatttc taaaaggagg tcaaaatttc 841 ctaattctgg taaatatgcc actacagctc gtaactcctt tattgtactt atcattttta 901 ctatatgttt tgttccctat catgcctttc gattcatcta catttcttca cagctaaatg 961 tatcatcttg ctactGGAAA GAAATTGTTC ACAAAACCAA TGAGATCATG CTGGTTCTCT 1021 CATCTTTCAA TAGCTGCTTA GATCCAGTCA TGTATTTCCT GATGTCCAGT AACATTCGCA 1081 AAATAATGTG CCAACTTCTT TTTAGACGAT TTCAAGGTGA ACCAAGTAGG AGTGAAAGCA 1141 CTTCAGAATT TAAACCAGGA TACTCCCTGC ATGATACATC TGTGGCAGTG AAAATACAGT 1201 CTAGTTCTAA AAGTACTGAC CCAGCTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1261 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1321 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACAATCAC CTTTTAATGA 1381 AATTTGTACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt