Construct: ORF TRCN0000492087
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015410.1_s317c1
- Derived from:
- ccsbBroadEn_03792
- DNA Barcode:
- ACCTACCTTCTCGAATATTAGTAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DAZ4 (57135)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492087
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57135 | DAZ4 | deleted in azoospermia 4 | NM_020420.3 | 100% | 100% | |
| 2 | human | 57055 | DAZ2 | deleted in azoospermia 2 | NM_001005786.2 | 92.8% | 92% | (many diffs) |
| 3 | human | 57054 | DAZ3 | deleted in azoospermia 3 | NM_020364.3 | 88.7% | 88.1% | (many diffs) |
| 4 | human | 1617 | DAZ1 | deleted in azoospermia 1 | XM_011531483.1 | 73.6% | 73.5% | 1_346del;347_348insT;1339_1410del |
| 5 | human | 57055 | DAZ2 | deleted in azoospermia 2 | NM_001005785.2 | 72.9% | 73% | 504T>C;994_1425del |
| 6 | human | 57055 | DAZ2 | deleted in azoospermia 2 | NM_020363.3 | 69.8% | 69.8% | 504T>C;994_1497del |
| 7 | human | 57135 | DAZ4 | deleted in azoospermia 4 | NM_001005375.2 | 67.2% | 67.1% | 1_496del;497_498insT;1489_1560del |
| 8 | human | 57135 | DAZ4 | deleted in azoospermia 4 | XM_011531510.1 | 60.9% | 60.9% | 0_1ins150;844_1347del |
| 9 | human | 57135 | DAZ4 | deleted in azoospermia 4 | XM_011531509.3 | 57.8% | 57.8% | 1_346del;347_348insT;1339_1842del |
| 10 | human | 1618 | DAZL | deleted in azoospermia like | NM_001351.4 | 56.2% | 42.5% | (many diffs) |
| 11 | human | 1617 | DAZ1 | deleted in azoospermia 1 | XM_011531482.2 | 56.1% | 56% | 1_841del;842_843insT;1834_1905del |
| 12 | human | 57135 | DAZ4 | deleted in azoospermia 4 | XM_005262602.1 | 53.8% | 53.8% | 1_496del;497_498insT;1489_1992del |
| 13 | human | 1618 | DAZL | deleted in azoospermia like | NM_001190811.1 | 53.5% | 40.4% | (many diffs) |
| 14 | human | 1617 | DAZ1 | deleted in azoospermia 1 | NM_004081.5 | 52.3% | 52.2% | 1_991del;992_993insT;1984_2055del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1236
- ORF length:
- 1170
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc tgctgcaaat cctgagactc caaactcaac catctccaga gaggccagca 121 cccagtcttc atcagctgca gctagccaag gctgggtgtt accagaaggc aaaatcgtgc 181 caaacactgt ttttgttggt ggaattgatg ctaggatgga tgaaactgag attggaagct 241 gctttggtag atacggttca gtgaaagaag tgaagataat cacgaatcga actggtgtgt 301 ccaaaggcta tggatttgtt tcgtttgtta atgacgtgga tgtccagaag atagtaggat 361 cacagataca tttccatggt aaaaagctga agctgggccc tgcaatcagg aaacaaaagt 421 tatgtgctcg tcatgtgcag ccacgtcctt tggtagttaa tcctcctcct ccaccacagt 481 ttcagaacgt ctggcggaat ccaaacactg aaacctacct gcagccccaa atcacgccga 541 atcctgtaac tcagcacgtt caggcttact ctgcttatcc acattcacca ggtcaggtca 601 tcactggatg tcagttgctt gtatataatt atcaggaata tcctacttat cccgattcag 661 catttcaggt caccactgga tatcagttgc ctgtatataa ttatcagcca tttcctgctt 721 atccaagatc accatttcag gtcactgctg gatatcagtt gcctgtatat aattatcagg 781 catttcctgc ttatccaaat tcaccatttc aagtcgccac tggatatcag ttccctgtat 841 acaattatca gccatttCCT GCTTATCCAA GTTCACCATT TCAGGTCACT GCTGGATATC 901 AGTTGCCTGT ATATAATTAT CAGGCATTTC CTGCTTATCC AAATTCACCA TTTCAAGTCG 961 CCACTGGATA TCAGTTCCCT GTATACAATT ATCAGGCATT TCCTGCTTAT CCAAATTCAC 1021 CAGTTCAGGT CACCACTGGA TATCAGTTGC CTGTATACAA TTATCAGGCA TTTCCTGCTT 1081 ATCCAAATTC AGCAGTTCAG GTCACCACTG GATATCAGTT CCATGTATAC AATTACCAGA 1141 TGCCACCGCA GTGCCCTGTT GGGGAGCAAA GGAGAAATCT GTGGACCGAA GCATACAAAT 1201 GGTGGTATCT TGTCTGTTTA ATCCAGAGAA GAGACTGCCC AACTTTCTTG TACAAAGTGG 1261 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1321 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1381 AACCTACCTT CTCGAATATT AGTAAACGCG TTAAGTCgac aatcaacctc tggattacaa 1441 aatttgtgaa agatt