Construct: ORF TRCN0000492107
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003806.1_s317c1
- Derived from:
- ccsbBroadEn_10263
- DNA Barcode:
- CCGCAATGGTCACGCTTGATCTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FRMD8P1 (83957)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492107
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 83786 | FRMD8 | FERM domain containing 8 | NM_031904.5 | 76% | 74.5% | (many diffs) |
| 2 | human | 83786 | FRMD8 | FERM domain containing 8 | NM_001300833.3 | 68.8% | 67.4% | (many diffs) |
| 3 | human | 83786 | FRMD8 | FERM domain containing 8 | NM_001300832.3 | 64.4% | 63.1% | (many diffs) |
| 4 | human | 83957 | FRMD8P1 | FERM domain containing 8 ps... | NR_033742.1 | 61.5% | 1_129del;1237_1800del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1176
- ORF length:
- 1107
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggacgggacg gaaggcagtg tcgggcagcc cggccccact gagcggtccc 121 accgaagcag cgtgtcctcc gtgggagccc gagcggctga cgtgctggta tacctggcag 181 atgacacggt ggtgcccctg gctgtggaga acctgccctc gctccgtgcc catgagctgc 241 actgcgccgt ccgcgaggtc ctgaagcttc cggacatcgc cctggatgtc ttcgcgctct 301 ggctggtctc ccctctgctg gaggtgcagc tgaaacccaa gaaccagccc tacaagctgg 361 gacgccagtg gccggagctg ctgctgcgct tcaccagtgc cccggacgac gacgtggcca 421 tggatgagcc tttcctgcag ttccgaagga acgtgttctt cccaaagggg cgggagctcc 481 agatccccga cgaggaggtc ctgcggctgc tctatgagga ggccaagggc aacgtgctgg 541 ctgcacggta cctgtgcgac gttgaggact gcgaggctct gggcgccctg gtgtgccgcg 601 tgcagctcgt gacctaccag cccgactggc cggcagcctg cgacctgagg gagaagctgg 661 actccttcct ccctgcccac ctctgtaagc ggggccaggg tctctttgct gccctccggg 721 gccgtggggc cagggccggg ccgggtgagc agagcctgct gaacgcctac cgcaaggtgc 781 aggaggtcag cagcgacggc gggtgtgagg ccgccctggg cacccactac cgcgcctacc 841 tccTCAAGTG CCACAAGCTG CCCTTCTATG GGTGTGCCTT CTTCCACGGT GAGGTTGACA 901 AGCCAGCCCA AGGCTTTTTG CACCGGGGTG GGCGCAAATC AGTTTCTGTG GCCATCAGCC 961 TGGAAGGTGT GCACGTCGTG GACAGCAGAG AGAAGCACGT CCTGCTGGGC CTGTGCTTCC 1021 AGGAGCTGTC GTGGGACCAC ACCTCCCCTG AGGAGGAGGA GCCCATCCTG TGGCTGGAGT 1081 TCGACAGGGA CAGCGGGGTC ACACCTGTCA ACAAGCTCCT CAAGATCTAC TCCAAGCAGG 1141 CCGAACTGAT GAGCAGCCTC ATTGAGTACT GCATCTTGCC AACTTTCTTG TACAAAGTGG 1201 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1261 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1321 ACCGCAATGG TCACGCTTGA TCTTAACGCG TTAAGTCgac aatcaacctc tggattacaa 1381 aatttgtgaa agatt